Phan Huyen T T, Ellwood Simon R, Hane James K, Ford Rebecca, Materne Michael, Oliver Richard P
Australian Centre for Nectrotropic Fungal Pathogens, State Agricultural Biotechnology Centre, Department of Health Sciences, Murdoch University, Perth, WA 6150, Australia.
Theor Appl Genet. 2007 Feb;114(3):549-58. doi: 10.1007/s00122-006-0455-3. Epub 2006 Nov 22.
The first predominantly gene-based genetic linkage map of lentil (Lens culinaris ssp. culinaris) was constructed using an F5 population developed from a cross between the cultivars Digger (ILL5722) and Northfield (ILL5588) using 79 intron-targeted amplified polymorphic (ITAP) and 18 genomic simple sequence repeat (SSR) markers. Linkage analysis revealed seven linkage groups (LGs) comprised of 5-25 markers that varied in length from 80.2 to 274.6 cM. The genome map spanned a total length of 928.4 cM. Clear evidence of a simple and direct macrosyntenic relationship between lentil and Medicago truncatula was observed. Sixty-six out of the 71 gene-based markers, which were previously assigned to M. truncatula genetic and physical maps, were found in regions syntenic between the Lens c. ssp. culinaris and M. truncatula genomes. However, there was evidence of moderate chromosomal rearrangements which may account for the difference in chromosome numbers between these two legume species. Eighteen common SSR markers were used to connect the current map with the most comprehensive and recent map that exists for lentil, providing the syntenic context of four important domestication traits. The composite map presented, anchored with orthologous markers mapped in M. truncatula, provides a strong foundation for the future use of genomic and genetic information in lentil genetic analysis and breeding.
利用从栽培品种迪格（ILL5722）和诺思菲尔德（ILL5588）杂交产生的F5群体，使用79个内含子靶向扩增多态性（ITAP）标记和18个基因组简单序列重复（SSR）标记，构建了首个主要基于基因的兵豆（Lens culinaris ssp. culinaris）遗传连锁图谱。连锁分析揭示了由5至25个标记组成的7个连锁群（LG），其长度在80.2至274.6厘摩之间变化。基因组图谱全长928.4厘摩。观察到兵豆与蒺藜苜蓿之间存在简单直接的宏观共线性关系的明确证据。在先前已被定位到蒺藜苜蓿遗传图谱和物理图谱上的71个基于基因的标记中，有66个在兵豆栽培种和蒺藜苜蓿基因组之间的共线区域中被发现。然而，有证据表明存在中度染色体重排，这可能解释了这两种豆科植物染色体数目的差异。使用18个常见的SSR标记将当前图谱与现有的最全面且最新的兵豆图谱相连接，提供了四个重要驯化性状的共线背景。所呈现的复合图谱以在蒺藜苜蓿中定位的直系同源标记为锚定，为未来在兵豆遗传分析和育种中利用基因组和遗传信息奠定了坚实基础。
