Ates Duygu, Aldemir Secil, Alsaleh Ahmad, Erdogmus Semih, Nemli Seda, Kahriman Abdullah, Ozkan Hakan, Vandenberg Albert, Tanyolac Bahattin
Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir, Turkey.
Department of Field Crops, Faculty of Agriculture, Cukurova University, Adana, Turkey.
PLoS One. 2018 Jan 19;13(1):e0191375. doi: 10.1371/journal.pone.0191375. eCollection 2018.
Lentil (Lens culinaris ssp. culinaris Medikus) is a diploid (2n = 2x = 14), self-pollinating grain legume with a haploid genome size of about 4 Gbp and is grown throughout the world with current annual production of 4.9 million tonnes.
A consensus map of lentil (Lens culinaris ssp. culinaris Medikus) was constructed using three different lentils recombinant inbred line (RIL) populations, including "CDC Redberry" x "ILL7502" (LR8), "ILL8006" x "CDC Milestone" (LR11) and "PI320937" x "Eston" (LR39).
The lentil consensus map was composed of 9,793 DArT markers, covered a total of 977.47 cM with an average distance of 0.10 cM between adjacent markers and constructed 7 linkage groups representing 7 chromosomes of the lentil genome. The consensus map had no gap larger than 12.67 cM and only 5 gaps were found to be between 12.67 cM and 6.0 cM (on LG3 and LG4). The localization of the SNP markers on the lentil consensus map were in general consistent with their localization on the three individual genetic linkage maps and the lentil consensus map has longer map length, higher marker density and shorter average distance between the adjacent markers compared to the component linkage maps.
This high-density consensus map could provide insight into the lentil genome. The consensus map could also help to construct a physical map using a Bacterial Artificial Chromosome library and map based cloning studies. Sequence information of DArT may help localization of orientation scaffolds from Next Generation Sequencing data.
小扁豆（Lens culinaris ssp. culinaris Medikus）是一种二倍体（2n = 2x = 14）、自花授粉的豆科作物，单倍体基因组大小约为4 Gbp，在全球范围内种植，目前年产量为490万吨。
利用三个不同的小扁豆重组自交系（RIL）群体构建了小扁豆（Lens culinaris ssp. culinaris Medikus）的整合图谱，包括“CDC Redberry”דILL7502”（LR8）、“ILL8006”דCDC Milestone”（LR11）和“PI320937”דEston”（LR39）。
小扁豆整合图谱由9793个DArT标记组成，总长度为977.47 cM，相邻标记间平均距离为0.10 cM，构建了7个连锁群，代表小扁豆基因组的7条染色体。整合图谱中没有大于12.67 cM的间隙，仅在LG3和LG4上发现5个间隙在12.67 cM至6.0 cM之间。SNP标记在小扁豆整合图谱上的定位总体上与其在三个单独遗传连锁图谱上的定位一致，与组成连锁图谱相比，小扁豆整合图谱具有更长的图谱长度、更高的标记密度和更短的相邻标记间平均距离。
这一高密度整合图谱有助于深入了解小扁豆基因组。该整合图谱还可帮助利用细菌人工染色体文库构建物理图谱以及基于图谱的克隆研究。DArT的序列信息可能有助于从下一代测序数据中定位定向支架。
