巴弗洛霉素导致的神经递质耗竭由囊泡周转所促进。
Neurotransmitter depletion by bafilomycin is promoted by vesicle turnover.
作者信息
Cavelier Pauline, Attwell David
机构信息
Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK.
出版信息
Neurosci Lett. 2007 Jan 29;412(2):95-100. doi: 10.1016/j.neulet.2006.10.040. Epub 2006 Nov 22.
Abstract
Accumulation of neurotransmitter into synaptic vesicles is powered by the vacuolar proton ATPase. We show here that, in brain slices, application of the H(+)-ATPase inhibitors bafilomycin or concanamycin does not efficiently deplete glutamatergic vesicles of transmitter unless vesicle turnover is increased. Simulations of vesicle energetics suggest either that bafilomycin and concanamycin act on the H(+)-ATPase from inside the vesicle, or that the vesicle membrane potential is maintained after the H(+)-ATPase is inhibited.
摘要
神经递质向突触囊泡的积累由液泡质子ATP酶提供能量。我们在此表明，在脑片中，除非囊泡周转率增加，否则应用H(+) -ATP酶抑制剂巴弗洛霉素或 concanamycin 并不能有效地耗尽谷氨酸能囊泡中的递质。囊泡能量学模拟表明，要么巴弗洛霉素和 concanamycin 从囊泡内部作用于H(+) -ATP酶，要么在H(+) -ATP酶被抑制后囊泡膜电位得以维持。
相似文献
1
Neurotransmitter depletion by bafilomycin is promoted by vesicle turnover.巴弗洛霉素导致的神经递质耗竭由囊泡周转所促进。
Neurosci Lett. 2007 Jan 29;412(2):95-100. doi: 10.1016/j.neulet.2006.10.040. Epub 2006 Nov 22.
2
Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool.突触小泡在自发状态下以及活动期间的循环属于同一个小泡池。
Nat Neurosci. 2007 Feb;10(2):145-7. doi: 10.1038/nn1831. Epub 2007 Jan 14.
3
Effects of reduced vesicular filling on synaptic transmission in rat hippocampal neurones.囊泡充盈减少对大鼠海马神经元突触传递的影响。
J Physiol. 2000 May 15;525 Pt 1(Pt 1):195-206. doi: 10.1111/j.1469-7793.2000.t01-1-00195.x.
4
Activity-dependent activation of presynaptic protein kinase C mediates post-tetanic potentiation.突触前蛋白激酶C的活动依赖性激活介导强直后增强。
Nat Neurosci. 2003 Jun;6(6):551-2. doi: 10.1038/nn1067.
5
Synaptic vesicles control the time course of neurotransmitter secretion via a Ca²+/H+ antiport.突触囊泡通过 Ca²⁺/H⁺反向转运来控制神经递质分泌的时程。
J Physiol. 2011 Jan 1;589(Pt 1):149-67. doi: 10.1113/jphysiol.2010.199224.
6
A single packet of transmitter does not saturate postsynaptic glutamate receptors.单个递质包不会使突触后谷氨酸受体饱和。
Neuron. 2002 May 16;34(4):613-21. doi: 10.1016/s0896-6273(02)00692-x.
7
Presynaptic Ca2+-activated K+ channels in glutamatergic hippocampal terminals and their role in spike repolarization and regulation of transmitter release.谷氨酸能海马终末中的突触前钙离子激活钾通道及其在动作电位复极化和递质释放调节中的作用。
J Neurosci. 2001 Dec 15;21(24):9585-97. doi: 10.1523/JNEUROSCI.21-24-09585.2001.
8
Distinct profiles of refilling of inhibitory neurotransmitters into presynaptic terminals projecting to spinal neurones in immature rats.幼鼠中向脊髓神经元投射的突触前终末中抑制性神经递质再填充的不同模式。
J Physiol. 2004 Oct 15;560(Pt 2):469-78. doi: 10.1113/jphysiol.2004.067017. Epub 2004 Aug 12.
9
Fast kinetics of exocytosis revealed by simultaneous measurements of presynaptic capacitance and postsynaptic currents at a central synapse.通过在中枢突触同时测量突触前电容和突触后电流揭示的快速胞吐动力学。
Neuron. 2001 Apr;30(1):171-82. doi: 10.1016/s0896-6273(01)00271-9.
10
Presynaptic HCN channels regulate vesicular glutamate transport.突触前HCN通道调节囊泡谷氨酸转运。
Neuron. 2014 Oct 22;84(2):340-6. doi: 10.1016/j.neuron.2014.08.046. Epub 2014 Sep 25.
引用本文的文献
1
Substrate recognition and allosteric regulation of synaptic vesicle glutamate transporter VGLUT2.突触囊泡谷氨酸转运体VGLUT2的底物识别与变构调节
Nat Struct Mol Biol. 2025 Jun 3. doi: 10.1038/s41594-025-01568-8.
2
The CHD family chromatin remodeling enzyme, Kismet, promotes both clathrin-mediated and activity-dependent bulk endocytosis.CHD 家族染色质重塑酶 Kismet 促进网格蛋白介导的和活动依赖性的大体积胞吞作用。
PLoS One. 2024 Mar 21;19(3):e0300255. doi: 10.1371/journal.pone.0300255. eCollection 2024.
3
The CD63 homologs, Tsp42Ee and Tsp42Eg, restrict endocytosis and promote neurotransmission through differential regulation of synaptic vesicle pools.CD63同源物Tsp42Ee和Tsp42Eg通过对突触小泡池的差异调节来限制内吞作用并促进神经传递。
Front Cell Neurosci. 2022 Aug 22;16:957232. doi: 10.3389/fncel.2022.957232. eCollection 2022.
4
Detection of Glutamate Encapsulated in Liposomes by Optical Trapping Raman Spectroscopy.利用光镊拉曼光谱法检测脂质体包裹的谷氨酸
ACS Omega. 2022 Feb 28;7(11):9701-9709. doi: 10.1021/acsomega.1c07206. eCollection 2022 Mar 22.
5
Spontaneous, synchronous zinc spikes oscillate with neural excitability and calcium spikes in primary hippocampal neuron culture.原代海马神经元培养中，自发、同步的锌峰与神经兴奋性和钙峰振荡。
J Neurochem. 2021 Jun;157(6):1838-1849. doi: 10.1111/jnc.15334. Epub 2021 Mar 14.
6
Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse.细胞外谷氨酸的不完全清除控制小脑突触的突触传递和整合。
Elife. 2021 Feb 22;10:e63819. doi: 10.7554/eLife.63819.
7
Non-canonical glutamate signaling in a genetic model of migraine with aura.遗传性有先兆偏头痛模型中的非经典谷氨酸信号转导。
Neuron. 2021 Feb 17;109(4):611-628.e8. doi: 10.1016/j.neuron.2020.11.018. Epub 2020 Dec 14.
8
Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes.线粒体通透性转换孔的瞬时开放诱导星形胶质细胞突起中的微区钙瞬变。
Neuron. 2017 Feb 8;93(3):587-605.e7. doi: 10.1016/j.neuron.2016.12.034. Epub 2017 Jan 26.
9
Mechanisms, pools, and sites of spontaneous vesicle release at synapses of rod and cone photoreceptors.视杆和视锥光感受器突触处自发囊泡释放的机制、库和位点。
Eur J Neurosci. 2016 Aug;44(3):2015-27. doi: 10.1111/ejn.13288. Epub 2016 Jun 22.
10
The V-ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery.V-ATPase 膜结构域是颗粒 pH 的感受器，它控制着胞吐机制。
J Cell Biol. 2013 Oct 28;203(2):283-98. doi: 10.1083/jcb.201303104.
本文引用的文献
1
The kinetics of synaptic vesicle reacidification at hippocampal nerve terminals.海马神经末梢突触囊泡再酸化的动力学
J Neurosci. 2006 Feb 22;26(8):2313-20. doi: 10.1523/JNEUROSCI.4425-05.2006.
2
The effect of simulated ischaemia on spontaneous GABA release in area CA1 of the juvenile rat hippocampus.模拟缺血对幼年大鼠海马体CA1区自发性γ-氨基丁酸释放的影响。
J Physiol. 2004 Dec 1;561(Pt 2):485-98. doi: 10.1113/jphysiol.2004.070490. Epub 2004 Sep 30.
3
The bafilomycin/concanamycin binding site in subunit c of the V-ATPases from Neurospora crassa and Saccharomyces cerevisiae.粗糙脉孢菌和酿酒酵母V-ATP酶亚基c中巴弗洛霉素/ concanamycin结合位点
J Biol Chem. 2004 Aug 6;279(32):33131-8. doi: 10.1074/jbc.M404638200. Epub 2004 Jun 4.
4
Sequential release of GABA by exocytosis and reversed uptake leads to neuronal swelling in simulated ischemia of hippocampal slices.通过胞吐作用和逆向摄取顺序释放γ-氨基丁酸（GABA）会导致海马切片模拟缺血时神经元肿胀。
J Neurosci. 2004 Apr 14;24(15):3837-49. doi: 10.1523/JNEUROSCI.5539-03.2004.
5
Distinct quantal features of AMPA and NMDA synaptic currents in hippocampal neurons: implication of glutamate spillover and receptor saturation.海马神经元中AMPA和NMDA突触电流的独特量子特征：谷氨酸溢出和受体饱和的影响。
Biophys J. 2003 Nov;85(5):3375-87. doi: 10.1016/S0006-3495(03)74757-2.
6
Development of rat CA1 neurones in acute versus organotypic slices: role of experience in synaptic morphology and activity.急性切片与器官型切片中大鼠CA1神经元的发育：经验在突触形态和活性中的作用
J Physiol. 2003 Jul 1;550(Pt 1):135-47. doi: 10.1113/jphysiol.2003.039099.
7
The SLC32 transporter, a key protein for the synaptic release of inhibitory amino acids.SLC32转运体，一种抑制性氨基酸突触释放的关键蛋白。
Pflugers Arch. 2004 Feb;447(5):756-9. doi: 10.1007/s00424-003-1091-2. Epub 2003 May 16.
8
Multiple modes of GABAergic inhibition of rat cerebellar granule cells.大鼠小脑颗粒细胞的多种GABA能抑制模式。
J Physiol. 2003 Apr 1;548(Pt 1):97-110. doi: 10.1113/jphysiol.2002.036459. Epub 2003 Feb 14.
9
Receptor occupancy limits synaptic depression at climbing fiber synapses.受体占有率限制了攀缘纤维突触处的突触抑制。
J Neurosci. 2003 Jan 15;23(2):377-83. doi: 10.1523/JNEUROSCI.23-02-00377.2003.
10
Proton pumping in the secretory pathway.分泌途径中的质子泵浦
J Membr Biol. 2001 Aug 1;182(3):159-69. doi: 10.1007/s00232-001-0040-2.
Zotero 插件