Fusayasu Emi, Kowa Hisanori, Takeshima Takao, Nakaso Kazuhiro, Nakashima Kenji
Department of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, 36-1 Nishi-cho, Yonago 683-8504, Japan.
Pain. 2007 Apr;128(3):209-214. doi: 10.1016/j.pain.2006.09.017. Epub 2006 Nov 22.
Substance P (SP), calcitonin gene-related peptide (CGRP), and angiotensin converting enzyme (ACE) may have roles in trigeminovascular nociceptive mechanisms. We investigated interictal levels of SP, CGRP, ACE activity, and their correlation, in a sample of migraineurs. Forty-one patients suffering from migraine with aura (MA), 54 without aura (MO), and 52 non-headache subjects (controls) participated in this study. Blood samples were collected from cubital veins. Plasma levels of SP and CGRP were measured by enzyme immunoassay. Plasma ACE activities were measured spectrophotometrically. SP levels in MA (6.6+/-3.7 pg/ml; mean+/-SD) and MO (6.6+/-3.2 pg/ml) were significantly higher than in controls (4.8+/-2.4 pg/ml) (P<0.01). CGRP levels in MA (18.8+/-8.8 pg/ml) and MO (19.1+/-9.4 pg/ml) were also significantly higher than in controls (13.4+/-4.4 pg/ml) (P<0.01). ACE activities in MA (34.6+/-19.0 U/l) were significantly higher than in MO (25.3+/-13.2 U/l) and controls (27.0+/-20.4 U/l) (P<0.05). There was a significant correlation between SP and CGRP levels (P<0.05). In MA, SP and CGRP showed a tendency toward positive correlation, which was not significant. There was a weak, but significant positive correlation between SP levels and ACE activities (P<0.01). However, a relationship between ACE activities and CGRP levels was not observed. The data suggest that SP, CGRP, and ACE are relevant to migraine pathophysiology, and that they may interact.
P物质（SP）、降钙素基因相关肽（CGRP）和血管紧张素转换酶（ACE）可能在三叉神经血管性伤害感受机制中发挥作用。我们在一组偏头痛患者中研究了SP、CGRP的发作间期水平、ACE活性及其相关性。41例伴有先兆偏头痛（MA）患者、54例无先兆偏头痛（MO）患者和52例无头痛受试者（对照组）参与了本研究。从肘静脉采集血样。采用酶免疫分析法测定血浆SP和CGRP水平。用分光光度法测定血浆ACE活性。MA组（6.6±3.7 pg/ml；均值±标准差）和MO组（6.6±3.2 pg/ml）的SP水平显著高于对照组（4.8±2.4 pg/ml）（P<0.01）。MA组（18.8±8.8 pg/ml）和MO组（19.1±9.4 pg/ml）的CGRP水平也显著高于对照组（13.4±4.4 pg/ml）（P<0.01）。MA组的ACE活性（34.6±19.0 U/l）显著高于MO组（25.3±13.2 U/l）和对照组（27.0±20.4 U/l）（P<0.05）。SP和CGRP水平之间存在显著相关性（P<0.05）。在MA组中，SP和CGRP呈正相关趋势，但不显著。SP水平与ACE活性之间存在微弱但显著的正相关（P<0.01）。然而，未观察到ACE活性与CGRP水平之间的关系。数据表明，SP、CGRP和ACE与偏头痛病理生理学相关，且它们可能相互作用。
