Strommenger Birgit, Schmidt Christiane, Werner Guido, Roessle-Lorch Beate, Bachmann Till T, Witte Wolfgang
Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
Mol Cell Probes. 2007 Jun;21(3):161-70. doi: 10.1016/j.mcp.2006.10.003. Epub 2006 Oct 19.
An oligonucleotide microarray was constructed for the rapid and sensitive molecular detection of antibiotic resistance determinants in Staphylococcus aureus. The array is equipped with oligonucleotide capture probes for the detection of 10 clinically and therapeutically relevant antibiotic resistance genes and -mutations (mecA, aacA-aphD, tetK, tetM, vat(A), vat(B), vat(C), erm(A), erm(C), grlA-mutation) as well as several control probes. A microarray concept was established including multiplexed PCR amplification, DNA labeling, hybridization and data processing. This concept was applied to clinical Staphylococcus aureus isolates and results were concordant with those from standard genotypic and phenotypic resistance testing. Our microarray concept offers rapid and accurate identification of antibiotic resistance profiles. It is easily expandable and thus can be adapted to changing clinical and epidemiological requirements in clinical diagnosis as well as in epidemiological studies.
构建了一种寡核苷酸微阵列，用于快速、灵敏地分子检测金黄色葡萄球菌中的抗生素耐药决定因素。该阵列配备了用于检测10个临床和治疗相关抗生素耐药基因及突变（mecA、aacA-aphD、tetK、tetM、vat(A)、vat(B)、vat(C)、erm(A)、erm(C)、grlA突变）的寡核苷酸捕获探针以及几个对照探针。建立了一个包括多重PCR扩增、DNA标记、杂交和数据处理的微阵列概念。该概念应用于临床金黄色葡萄球菌分离株，结果与标准基因型和表型耐药性检测结果一致。我们的微阵列概念能够快速、准确地鉴定抗生素耐药谱。它易于扩展，因此可适应临床诊断以及流行病学研究中不断变化的临床和流行病学需求。
