Tammi R, Tammi M
Department of Anatomy, University of Kuopio, Finland.
Exp Cell Res. 1991 Aug;195(2):524-7. doi: 10.1016/0014-4827(91)90405-j.
The rates of keratinocyte proliferation and synthesis of Hyaluronan (HA) were studied in human whole-skin organ culture by labeling with [6-3H]glucosamine and [3H]thymidine, respectively, to reveal possible correlations between the two functions of the cell. HA distribution in epidermis was examined by staining with a specific probe prepared from cartilage proteoglycan. The keratinocyte proliferation rate was low on the first 2 culture days, but showed a tenfold increase on the third and fourth days while the synthesis of HA proceeded at a relatively stable level throughout the same period. The most intensive staining of HA occurred in the uppermost spinous cell layer, whereas mitotic cells resided in the basal and suprabasal layers. The keratinocytes under various stages of mitosis were surrounded by a HA staining not more intense than that around nondividing basal cells, but a thick pad of HA appeared rapidly between the daughter cells. These findings suggest that newly synthesized HA is associated with the separation of keratinocytes following mitosis but the majority of the synthesis and content of HA in epidermis is involved in other keratinocyte activities such as maintenance of the extracellular space and cell--cell interactions during migration and differentiation.
通过分别用[6-³H]氨基葡萄糖和[³H]胸腺嘧啶核苷标记，在人全皮肤器官培养中研究角质形成细胞的增殖率和透明质酸（HA）的合成，以揭示细胞这两种功能之间可能的相关性。用从软骨蛋白聚糖制备的特异性探针染色来检查表皮中HA的分布。角质形成细胞增殖率在培养的前2天较低，但在第3天和第4天增加了10倍，而HA的合成在同一时期以相对稳定的水平进行。HA染色最强烈的部位出现在最上层的棘细胞层，而有丝分裂细胞位于基底层和基底上层。处于有丝分裂各个阶段的角质形成细胞周围的HA染色强度不比未分裂的基底细胞周围的染色强度更强，但在子细胞之间迅速出现了一层厚厚的HA垫。这些发现表明，新合成的HA与有丝分裂后角质形成细胞的分离有关，但表皮中HA的大部分合成和含量参与了其他角质形成细胞活动，如在迁移和分化过程中维持细胞外空间以及细胞间相互作用。
