Maroney Patricia A, Yu Yang, Fisher Jesse, Nilsen Timothy W
Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University School of Medicine, W127 10900 Euclid Avenue, Cleveland, Ohio 44106-4973, USA.
Nat Struct Mol Biol. 2006 Dec;13(12):1102-7. doi: 10.1038/nsmb1174. Epub 2006 Nov 26.
MicroRNAs (miRNAs) regulate gene expression post-transcriptionally by binding the 3' untranslated regions of target mRNAs. We examined the subcellular distribution of three miRNAs in exponentially growing HeLa cells and found that the vast majority are associated with mRNAs in polysomes. Several lines of evidence indicate that most of these mRNAs, including a known miRNA-regulated target (KRAS mRNA), are actively being translated.
微小RNA（miRNAs）通过与靶标mRNA的3'非翻译区结合，在转录后水平调控基因表达。我们检测了三种miRNA在指数生长期HeLa细胞中的亚细胞分布，发现绝大多数miRNA与多核糖体中的mRNA相关联。多项证据表明，这些mRNA中的大多数，包括一个已知的受miRNA调控的靶标（KRAS mRNA），正在被积极翻译。
