Friis Anne K, Heimann Axel C, Jakobsen Rasmus, Albrechtsen Hans-Jørgen, Cox Evan, Bjerg Poul L
Institute of Environment & Resources, Technical University of Denmark, Building 115, Bygningstorvet, DK-2800 Kgs. Lyngby, Denmark.
Water Res. 2007 Jan;41(2):355-64. doi: 10.1016/j.watres.2006.09.026. Epub 2006 Nov 28.
Temperature dependence of reductive trichloroethene (TCE) dechlorination was investigated in an enrichment culture (KB-1), using lactate or propionate as electron donors at a temperature interval from 4 to 60 degrees C. Dechlorination was complete to ethene at temperatures between 10 and 30 degrees C (lactate-amended) and between 15 and 30 degrees C (propionate-amended). Dechlorination stalled at cis-1,2-dichloroethene (cDCE) at 4 degrees C (lactate-amended), at and below 10 degrees C (propionate-amended), and at 40 degrees C with both electron donors. No dechlorination of TCE was observed at 50 and 60 degrees C. Concentrations of Dehalococcoides had increased or remained constant after 15 days of incubation at temperatures involving complete dechlorination to ethene. Temperature dependence of dechlorination rates was compared using zero order degradation kinetics and a Monod growth-rate model for multiple electron acceptor usage with competition. Maximum growth rates (mu) and zero order degradation rates were highest for TCE dechlorination at 30 degrees C with lactate as substrate (mu(TCE) of 7.00+/-0.14 days(-1)). In general, maximum growth rates and dechlorination rates of TCE were up to an order of magnitude higher than rates for utilization of cis-dichloroethene (cDCE, mu(c)(DCE) up to 0.17+/-0.02 days(-1)) and vinyl chloride (VC, mu(VC) up to 0.52+/-0.09 days(-1)). Temperature dependence of maximum growth rates and degradation rates of cDCE and VC were similar and highest at 15-30 degrees C, with growth rates on cDCE being lower than on VC. This study demonstrates that bioaugmentation of chlorinated ethene sites may be more efficient at elevated temperatures.
在富集培养物（KB-1）中，以乳酸盐或丙酸盐作为电子供体，在4至60摄氏度的温度区间内研究了还原性三氯乙烯（TCE）脱氯的温度依赖性。在10至30摄氏度（添加乳酸盐）和15至30摄氏度（添加丙酸盐）的温度下，脱氯反应完全进行至乙烯。在4摄氏度（添加乳酸盐）、10摄氏度及以下（添加丙酸盐）以及40摄氏度（两种电子供体）时，脱氯反应在顺式-1,2-二氯乙烯（cDCE）处停滞。在50和60摄氏度时未观察到TCE的脱氯现象。在温度下培养15天后，若脱氯反应完全进行至乙烯，则脱卤球菌的浓度会增加或保持不变。使用零级降解动力学和用于多电子受体利用及竞争的莫诺德生长速率模型，比较了脱氯速率的温度依赖性。以乳酸盐为底物时，30摄氏度下TCE脱氯的最大生长速率（μ）和零级降解速率最高（μ（TCE）为7.00±0.14天⁻¹）。一般来说，TCE的最大生长速率和脱氯速率比顺式二氯乙烯（cDCE，μ（c）（DCE）最高为0.17±0.02天⁻¹）和氯乙烯（VC，μ（VC）最高为0.52±0.09天⁻¹）的利用速率高一个数量级。cDCE和VC的最大生长速率和降解速率的温度依赖性相似，在15至30摄氏度时最高，cDCE的生长速率低于VC。本研究表明，在升高的温度下，氯化乙烯场地的生物强化可能更有效。
