el-Mohamady H, Basta M T, Seddek M N, Helmy H, al-Hilaly E, Attallah A M, Ghoneim M A
Urology and Nephrology Center, Mansoura University, Egypt.
Urol Res. 1991;19(2):145-50. doi: 10.1007/BF00368194.
The main distinctive feature of carcinoma in schistosomal bladder is keratinized squamous cell carcinoma. Keratins/cytokeratins constitute a multigeneic family of structurally related polypeptide markers for the malignant state of epithelial cells. A monoclonal antibody (UNME/K1) regognizing keratins associated with squamous cell carcinoma of the human urinary bladder was generated at the Urology and Nephrology Center, Mansoura, Egypt (UNME), by fusion of spleenocytes from a BALB/c mouse immunized with a keratin extract (K1) of human squamous cell carcinoma and P3X63Ag8/U1 syngeneic myeloma cells. UNME/K1 was purified by a protein-A affinity column and was of the IgG2a type, as determined by immunoelectrophoresis and gel diffusion techniques. When tested against keratins of different types of urinary bladder tumors using enzym linked immunosorbent assay (ELISA), UNME/K1 reacted only with the high molecular weight keratin of squamous cell carcinoma and showed selectivity towards specific histopathological grades of tumors.
血吸虫性膀胱中的癌的主要显著特征是角化鳞状细胞癌。角蛋白/细胞角蛋白构成了一个结构相关的多基因家族，是上皮细胞恶性状态的多肽标志物。埃及曼苏拉泌尿与肾病中心（UNME）通过将用人类鳞状细胞癌角蛋白提取物（K1）免疫的BALB/c小鼠的脾细胞与P3X63Ag8/U1同基因骨髓瘤细胞融合，产生了一种识别与人类膀胱鳞状细胞癌相关角蛋白的单克隆抗体（UNME/K1）。UNME/K1通过蛋白A亲和柱纯化，通过免疫电泳和凝胶扩散技术确定其为IgG2a型。当使用酶联免疫吸附测定（ELISA）针对不同类型膀胱肿瘤的角蛋白进行检测时，UNME/K1仅与鳞状细胞癌的高分子量角蛋白发生反应，并对特定组织病理学分级的肿瘤表现出选择性。
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