Gigi O, Geiger B, Eshhar Z, Moll R, Schmid E, Winter S, Schiller D L, Franke W W
EMBO J. 1982;1(11):1429-37. doi: 10.1002/j.1460-2075.1982.tb01334.x.
A monoclonal antibody derived from a mouse immunized with bovine epidermal prekeratin has been characterized by its binding to cytoskeletal polypeptides separated by one- or two-dimensional gel electrophoresis and by immunofluorescence microscopy. This antibody (KG 8.13) binds to a determinant present in a large number of human cytokeratin polypeptides, notably some polypeptides (Nos. 1, 5, 6, 7, and 8) of the 'basic cytokeratin subfamily' defined by peptide mapping, as well as a few acidic cytokeratins such as the epidermis-specific cytokeratins Nos. 10 and 11 and the more widespread cytokeratin No. 18. This antibody reacts specifically with a wide variety of epithelial tissues and cultured epithelial cells, in agreement with previous findings that at least one polypeptide of the basic cytokeratin subfamily is present in all normal and neoplastic epithelial cells so far examined. The antibody also reacts with corresponding cytokeratin polypeptides in a broad range of species including man, cow, chick, and amphibia but shows only limited reactivity with only a few rodent cytokeratins. The value of this broad-range monoclonal antibody, which apparently recognizes a stable cytokeratin determinant ubiquitous in human epithelia, for the immunohistochemical identification of epithelia and carcinomas is discussed.
一种由用牛表皮前角蛋白免疫的小鼠产生的单克隆抗体，已通过其与经一维或二维凝胶电泳分离的细胞骨架多肽的结合以及免疫荧光显微镜检查进行了表征。该抗体（KG 8.13）与大量人类细胞角蛋白多肽中存在的一个决定簇结合，特别是肽图谱定义的“碱性细胞角蛋白亚家族”中的一些多肽（第1、5、6、7和8号），以及一些酸性细胞角蛋白，如表皮特异性细胞角蛋白第10和11号以及分布更广的细胞角蛋白第18号。该抗体与多种上皮组织和培养的上皮细胞发生特异性反应，这与之前的研究结果一致，即到目前为止，在所有检查过的正常和肿瘤上皮细胞中至少存在一种碱性细胞角蛋白亚家族的多肽。该抗体还与包括人、牛、鸡和两栖动物在内的多种物种中的相应细胞角蛋白多肽发生反应，但仅与少数啮齿动物细胞角蛋白表现出有限的反应性。讨论了这种广泛反应的单克隆抗体在免疫组织化学鉴定上皮组织和癌方面的价值，该抗体显然识别一种在人类上皮中普遍存在的稳定细胞角蛋白决定簇。
