Tungekar M F, Gatter K C, Al-Adnani M S
Department of Pathology, Faculty of Medicine, Kuwait University.
J Clin Pathol. 1988 Dec;41(12):1288-96. doi: 10.1136/jcp.41.12.1288.
Expression of low and high molecular weight cytokeratin proteins was investigated immunohistochemically in a variety of transitional and squamous epithelial lesions of the urinary tract with and without schistosomiasis. The monoclonal antibodies used were CAM 5.2 and NCL5D3 for low, PK 63 and 121 for high, and MAK 6 for a broad range of intermediate molecular weight cytokeratins. On staining with CAM 5.2 and NCL5D3, urothelial hyperplasias (n = 12) and grades 1 (n = 5) and 2 (n = 10) papillary transitional cell carcinomas showed labelling patterns quite distinct from carcinoma in situ (n = 4) and non-papillary grades 2 (n = 6) and 3 tumours (n = 3). Among squamous lesions only focal positivity was obtained in 14 of 22 moderate to poorly differentiated squamous cell carcinomas. By contrast, PK 63 and 121 stained squamous lesions exclusively. MAK 6 stained the whole range of urothelial and squamous lesions with the exception of squamous metaplasias. Polyclonal antikeratin adequately labelled spindle cell areas of high grade tumours. The distinctive staining patterns given by these or similar antibodies may help in the identification of squamous metaplasia and in diagnosing tumours of the urothelium.
采用免疫组织化学方法，研究了低分子量和高分子量细胞角蛋白蛋白在伴有或不伴有血吸虫病的各种泌尿系统移行上皮和鳞状上皮病变中的表达情况。所用的单克隆抗体中，CAM 5.2和NCL5D3用于检测低分子量细胞角蛋白，PK 63和121用于检测高分子量细胞角蛋白，MAK 6用于检测广泛的中等分子量细胞角蛋白。用CAM 5.2和NCL5D3染色时，尿路上皮增生（n = 12）以及1级（n = 5）和2级（n = 10）乳头状移行细胞癌的标记模式与原位癌（n = 4）以及2级（n = 6）和3级非乳头状肿瘤（n = 3）明显不同。在鳞状病变中，22例中至低分化鳞状细胞癌中只有14例出现局灶性阳性。相比之下，PK 63和121仅对鳞状病变染色。MAK 6对除鳞状化生外的所有尿路上皮和鳞状病变均有染色。多克隆抗角蛋白能充分标记高级别肿瘤的梭形细胞区域。这些抗体或类似抗体所呈现的独特染色模式可能有助于鳞状化生的识别以及尿路上皮肿瘤的诊断。
