造纸工业流程及最终产品中有色沉积物形成的嗜热栖热菌属的检测与定量分析。
Detection and quantitation of colored deposit-forming Meiothermus spp. in paper industry processes and end products.
作者信息
Ekman Jaakko, Kosonen Mirva, Jokela Sanna, Kolari Marko, Korhonen Päivi, Salkinoja-Salonen Mirja
机构信息
Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, Helsinki, 00014 Finland.
出版信息
J Ind Microbiol Biotechnol. 2007 Mar;34(3):203-11. doi: 10.1007/s10295-006-0187-z. Epub 2006 Nov 28.
Colored biofilms cause problems in paper industry. In this work we used real-time PCR to detect and to quantitate members of the genus Meiothermus from the process samples and end products from 24 machines manufacturing pulp, paper and board in four countries. The results obtained from 200 samples showed the importance of members of the genus Meiothermus as ubiquitous biofoulers in paper machines. This genus was the dominant biofouler in some mills. From < or =10(4) to 10(11) copies of Meiothermus 16S rRNA genes were found per gram of process deposit (wet weight). Meiothermus spp. were found in paper and board products with colored defects and connection between deposit-forming microbes and end-product spots was shown. 16S rRNA gene sequences of 29 biofilm producing bacterial isolates from different mills were determined. Based on sequence data, 25 of the isolates were assigned to the genus Meiothermus, with Meiothermus silvanus and M. ruber as the most frequent species.
有色生物膜给造纸工业带来了问题。在这项研究中，我们使用实时聚合酶链反应（PCR）来检测和定量来自四个国家24家生产纸浆、纸张和纸板的机器的过程样品和最终产品中的嗜热栖热菌属成员。从200个样品中获得的结果表明，嗜热栖热菌属成员作为造纸机中普遍存在的生物污垢物的重要性。在一些工厂中，该属是主要的生物污垢物。每克过程沉积物（湿重）中发现嗜热栖热菌16S rRNA基因的拷贝数从≤10⁴到10¹¹不等。在有颜色缺陷的纸张和纸板产品中发现了嗜热栖热菌属物种，并且显示出形成沉积物的微生物与最终产品斑点之间的联系。测定了来自不同工厂的29株产生物膜细菌分离株的16S rRNA基因序列。根据序列数据，25株分离株被归为嗜热栖热菌属，其中嗜热栖热栖热菌和嗜热栖热红菌是最常见的物种。
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