Chung A P, Rainey F, Nobre M F, Burghardt J, da Costa M S
Departamento de Bioquímica, Universidade de Coimbra, Portugal.
Int J Syst Bacteriol. 1997 Oct;47(4):1225-30. doi: 10.1099/00207713-47-4-1225.
Strains of Meiothermus cerbereus sp. nov. were isolated from the hot springs within the Geysir geothermal area of Iceland. The strains of Meiothermus cerbereus produce red-orange-pigmented colonies, have an optimum growth temperature of about 55 degrees C, and have higher levels of 3-OH fatty acids than the strains of the other species of the genus Meiothermus. These strains, unlike all other strains of the species of the genus Meiothermus examined previously, required cysteine, thiosulfate, or thioglycolate for growth in liquid Thermus medium, but not in the corresponding medium solidified with agar. Several strains belonging to Meiothermus silvanus, isolated from Geysir, also required reduced sulfur compounds for growth in liquid medium, leading to the hypothesis that this requirement is not a taxonomic characteristic of the new species. The new species represented by strains GY-1T and GY-5 can be distinguished from the other species of the genus Meiothermus by biochemical characteristics, fatty acid composition, DNA-DNA reassociation values, and 16S ribosomal DNA sequence. The type strain for Meiothermus cerbereus is GY-1 (= DSM 11376).
新种梅氏嗜热栖热菌菌株是从冰岛间歇泉地热区的温泉中分离出来的。梅氏嗜热栖热菌菌株产生红橙色菌落，最适生长温度约为55℃，且3-羟基脂肪酸水平高于梅氏嗜热栖热菌属其他物种的菌株。与之前检测的梅氏嗜热栖热菌属所有其他菌株不同，这些菌株在液体嗜热栖热菌培养基中生长需要半胱氨酸、硫代硫酸盐或巯基乙酸盐，但在相应的琼脂固化培养基中则不需要。从间歇泉分离出的几株属于林地嗜热栖热菌的菌株在液体培养基中生长也需要还原态硫化合物，这导致一种假设，即这种需求并非新物种的分类特征。以菌株GY-1T和GY-5为代表的新物种可通过生化特性、脂肪酸组成、DNA-DNA重结合值和16S核糖体DNA序列与梅氏嗜热栖热菌属的其他物种区分开来。梅氏嗜热栖热菌的模式菌株是GY-1（=DSM 11376）。
