Yu Michael, de Carvalho Luiz Pedro Sorio, Sun Guangxing, Blanchard John S
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
J Am Chem Soc. 2006 Dec 6;128(48):15356-7. doi: 10.1021/ja066298w.
The Gcn5-related N-acetyltransferases (GNAT) comprise one of the largest enzyme superfamilies, with over 10 000 known members represented in all kingdoms of life. ChloroacetylCoenzymeA was prepared and demonstrated to be a substrate for several GNAT members. ChloroacetylCoA (ClAcCoA) is used by the Hat1 histone acetyltransferase to correctly acetylate histone H4 in a mixture of histone proteins. Chloroacetylation can be assessed by the subsequent reaction of the chloroacetylated product with thiol-containing compounds, including those with fluorescent or affinity (His8) tags. The bacterial RimL N-acetyltransferase also uses ClAcCoA to chloroacetyl the alpha-amino group of its cognate substrate, the ribosomal L12 protein, and this reaction can be observed in crude extracts. ChloroacetylCoA is a reagent that can be used to identify the unknown substrate(s) for this large family of functionally uncharacterized enzymes.
与Gcn5相关的N - 乙酰转移酶（GNAT）构成了最大的酶超家族之一，在所有生物界中已知成员超过10000个。制备了氯乙酰辅酶A，并证明它是几种GNAT成员的底物。Hat1组蛋白乙酰转移酶使用氯乙酰辅酶A（ClAcCoA）在组蛋白混合物中正确地乙酰化组蛋白H4。氯乙酰化可以通过氯乙酰化产物与含硫醇化合物（包括带有荧光或亲和（His8）标签的化合物）的后续反应来评估。细菌RimL N - 乙酰转移酶也使用ClAcCoA对其同源底物核糖体L12蛋白的α - 氨基进行氯乙酰化，并且该反应可以在粗提物中观察到。氯乙酰辅酶A是一种试剂，可用于鉴定这一功能未明确的酶大家族的未知底物。
