Chen L H, Emory S A, Bricker A L, Bouvet P, Belasco J G
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
J Bacteriol. 1991 Aug;173(15):4578-86. doi: 10.1128/jb.173.15.4578-4586.1991.
The 5' untranslated region (UTR) of the Escherichia coli ompA transcript functions in vivo as a growth rate-regulated mRNA stabilizer. The secondary structure of this mRNA segment has been determined by a combination of three methods: phylogenetic analysis, in vitro probing with a structure-specific RNase, and methylation by dimethylsulfate in vivo and in vitro. These studies reveal that despite extensive sequence differences, the 5' UTRs of the ompA transcripts of E. coli, Serratia marcescens, and Enterobacter aerogenes can fold in a remarkably similar fashion. Furthermore, the Serratia and Enterobacter ompA 5' UTRs function as effective mRNA stabilizers in E. coli. Stabilization of mRNA by the Serratia ompA 5' UTR is growth rate dependent. These findings indicate that the features of the ompA 5' UTR responsible for its ability to stabilize mRNA in a growth rate-regulated manner are to be found among the structural similarities shared by these diverse evolutionary variants.
大肠杆菌ompA转录本的5'非翻译区（UTR）在体内作为一种受生长速率调节的mRNA稳定剂发挥作用。该mRNA片段的二级结构已通过三种方法相结合来确定：系统发育分析、用结构特异性核糖核酸酶进行体外探测以及在体内和体外通过硫酸二甲酯进行甲基化。这些研究表明，尽管存在广泛的序列差异，但大肠杆菌、粘质沙雷氏菌和气肠杆菌ompA转录本的5' UTR能够以非常相似的方式折叠。此外，粘质沙雷氏菌和气肠杆菌的ompA 5' UTR在大肠杆菌中作为有效的mRNA稳定剂发挥作用。粘质沙雷氏菌ompA 5' UTR对mRNA的稳定作用取决于生长速率。这些发现表明，负责以生长速率调节方式稳定mRNA的ompA 5' UTR的特征可以在这些不同进化变体共有的结构相似性中找到。
