Barbone F P, Leibowitz M J
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Department of Molecular Genetics and Microbiology, Piscataway 08854-5635.
J Gen Virol. 1991 Jul;72 ( Pt 7):1755-60. doi: 10.1099/0022-1317-72-7-1755.
The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15 degrees C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.
酿酒酵母的细胞质遗传杀伤病毒通过在受感染细胞的细胞质中产生的明显无帽病毒转录本来表达其双链RNA基因组。这种天然温度敏感病毒的病毒粒子可以添加到未感染酵母细胞的无细胞翻译提取物中，从而在体外15摄氏度下引发病毒转录和翻译偶联的反应。在这个反应中，核苷酸被掺入到M和L-A双链RNA片段的全长转录本中，掺入基因组RNA的水平较低。此外，还观察到核苷酸掺入到一种较小的RNA物种中，该物种与M或L-A没有序列相关性。
