Bong M, Chakrabarti A, Banik N, Hogan E L, Kanoh M, Wiggins R C, Konat G
Department of Neurology, Medical University of South Carolina, Charleston 29425.
Metab Brain Dis. 1991 Mar;6(1):7-17. doi: 10.1007/BF01000381.
Rat glioma C6 cells were stably transfected with a pSV3-neo plasmid containing SV40 T antigen gene, and geniticin-resistant transfectants (designated C6T cells) were cloned. The C6T cells grew as well-defined foci of cells showing squamous or irregular morphology. The doubling time for transfected cells was reduced by approximately 40% as compared to control C6 cells. The transfection with T-antigen also affected the expression of genes coding for structural myelin proteins and for myelin-associated enzymes. The steady-state level of proteolipid protein (PLP)-specific mRNA in C6T cells was 44% lower than in parental C6 cells. On the other hand, the transfection upregulated the expression of myelin-associated glycoprotein (MAG) by 153%. The activity of 2':3' cyclic AMP phosphodiesterase (CNP) was increased by approximately 80% in the C6T cells as compared to untransfected, control cells. The activity of calcium-activated neutral proteinase (CANP) was also significantly elevated in the transfectants by approximately 50% and 220% for millimolar and micromolar form respectively. The results indicate that T antigen affects the expression of myelin genes, although, individual genes appear to be differently regulated implying the existence of several independent regulatory mechanisms.
将含有SV40 T抗原基因的pSV3-neo质粒稳定转染大鼠胶质瘤C6细胞，并克隆出对遗传霉素耐药的转染子（命名为C6T细胞）。C6T细胞生长为形态呈鳞状或不规则的界限分明的细胞集落。与对照C6细胞相比，转染细胞的倍增时间缩短了约40%。用T抗原转染还影响了编码结构性髓鞘蛋白和髓鞘相关酶的基因的表达。C6T细胞中蛋白脂蛋白（PLP）特异性mRNA的稳态水平比亲代C6细胞低44%。另一方面，转染使髓鞘相关糖蛋白（MAG）的表达上调了153%。与未转染的对照细胞相比，C6T细胞中2':3'环磷酸腺苷磷酸二酯酶（CNP）的活性增加了约80%。转染子中钙激活中性蛋白酶（CANP）的活性也显著升高，毫摩尔形式和微摩尔形式分别升高了约50%和220%。结果表明，T抗原影响髓鞘基因的表达，尽管个别基因似乎受到不同的调控，这意味着存在几种独立的调控机制。
