Sutcliffe J G, Milner R J, Shinnick T M, Bloom F E
Cell. 1983 Jul;33(3):671-82. doi: 10.1016/0092-8674(83)90010-7.
From the nucleotide sequences of three cDNA clones of rat brain-specific mRNAs, we deduced the partial amino acid sequences of two previously unknown proteins. We raised antisera to synthetic peptides mimicking short regions of these putative brain-specific proteins, and used these sera in immunocytochemical studies to localize each protein in the brain. One protein is found in large neurons throughout the brain, asymmetrically distributed toward the dendritic pole of the cell cytoplasm, suggesting involvement in the synthesis and/or directional transport of dendritic substances. The sequence of the second protein contains pairs of basic residues similar to precursors for neurotransmitters. The protein is located in (and may be the precursor for the neurotransmitter of) a novel fiber network of major extent with ramifications in cerebellum, hippocampus, and cortex, and in cell bodies in the brain stem, hypothalamus, and caudate nucleus. Our approach provides a direct method for characterizing rare brain molecules which may not have been anticipated and for which there are no known functional assays.
从大鼠脑特异性mRNA的三个cDNA克隆的核苷酸序列中，我们推导了两种先前未知蛋白质的部分氨基酸序列。我们制备了针对模拟这些假定的脑特异性蛋白质短区域的合成肽的抗血清，并将这些血清用于免疫细胞化学研究，以在脑中定位每种蛋白质。一种蛋白质存在于整个大脑的大神经元中，不对称地分布于细胞质的树突极，提示其参与树突物质的合成和/或定向运输。第二种蛋白质的序列包含与神经递质前体相似的碱性残基对。该蛋白质位于一个广泛的新型纤维网络中（可能是该神经递质的前体），该网络在小脑、海马体和皮质有分支，在脑干、下丘脑和尾状核的细胞体中也有分布。我们的方法为表征可能未曾预料到且尚无已知功能检测方法的罕见脑分子提供了一种直接方法。
