[通过免疫印迹法对结核分枝杆菌抗原决定簇抗体反应谱的表征]

[The characterization of the spectrum of the antibody response to Mycobacterium tuberculosis antigenic determinants by immunoblotting].

作者信息

Kiroshka V S, Shinkareva T I, Litvinov V I, Chernousova L N, Ginda S S, Gil'burd B Sh

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1991 Jan(1):61-3.

Abstract

The spectrum of antibody response to M. tuberculosis antigenic determinants H37Rv and M. bovis antigenic determinants BCG was studied in serum samples from 33 healthy donors and 31 patients with infiltrative pulmonary tuberculosis by the method of immunoblotting. The study revealed that most frequently tuberculosis patients showed response to Ag-H37Rv with molecular weights of 52, 39, 35, 21, 31, 68 kD (44.4-22.2%) and Ag-BCG with molecular weights 60, 58, 50, 25, 54, 70 kD. (33.3-22.2%). By month 9 of effective chemotherapy binding predominantly with Ag-H37Rv determinants of 31, 62, 35, 75, 56, 28, 19, 5, 13 kD (75-37.5%) and Ag-BCG determinants of 13, 34, 38, 44, 19, 36, 45, 52, 58, 60 70 kD (37.5-25%) were registered. Some differences in the spectra of antibody response to Ag-H37Rv and Ag-BCG determinants were noted.

摘要

采用免疫印迹法，对33名健康供体和31名浸润性肺结核患者的血清样本中针对结核分枝杆菌抗原决定簇H37Rv和牛分枝杆菌抗原决定簇卡介苗（BCG）的抗体反应谱进行了研究。研究发现，肺结核患者最常出现的反应是针对分子量为52、39、35、21、31、68kD的Ag-H37Rv（44.4%-22.2%）和分子量为60、58、50、25、54、70kD的Ag-BCG（33.3%-22.2%）。在有效化疗的第9个月时，主要与分子量为31、62、35、75、56、28、19、5、13kD的Ag-H37Rv决定簇（75%-37.5%）以及分子量为13、34、38、44、19、36、45、52、58、60、70kD的Ag-BCG决定簇（37.5%-25%）结合的情况被记录下来。观察到针对Ag-H37Rv和Ag-BCG决定簇的抗体反应谱存在一些差异。