Litvinov V I, Chernousova L N, Kalinina O A, Safonova S G, Dem'ianenko N V, Kulikovskaia N V, Kapina M A
Probl Tuberk. 1997(6):40-2.
Antibodies to Mycobacterium tuberculosis antigens H37Rv and reverse strains previously isolated from patients with sarcoidosis with granular isolates were determined in 50 patients with sarcoidosis (including 16 patients isolating granular types) and 56 patients with tuberculosis, by using ELISA and immunoblotting. Serum antibodies from patients with sarcoidosis were ascertained to more commonly react in ELISA with the antigen (ultrasound disintegrant (USDs) obtained from reverse mycobacteria isolated (initially) from patients with sarcoidosis (AGS) than with the USD of the M. tuberculosis H37Rv (AGT) and, on the contrary, serum antibodies from patients with tuberculosis more frequently reacted with the M. tuberculosis H37Rv. The spectrum of serum antibodies from patients with sarcoidosis greatly differed at immunoblotting with AGS and AGT. There was most commonly a reaction with the antigenic determinants 79, 27, 30, and 50 kDa to AGS and that of the determinants 17, 35, 32 kDa to AGT.
采用酶联免疫吸附测定（ELISA）和免疫印迹法，对50例结节病患者（包括16例分离出颗粒型菌株的患者）和56例结核病患者，检测其针对结核分枝杆菌抗原H37Rv及先前从结节病患者分离出的颗粒型菌株的反向菌株的抗体。结果发现，结节病患者血清抗体在ELISA中更常与从结节病患者（AGS）分离出的反向分枝杆菌获得的抗原（超声崩解产物（USDs））发生反应，而非与结核分枝杆菌H37Rv的USD（AGT）发生反应；相反，结核病患者血清抗体更常与结核分枝杆菌H37Rv发生反应。在对AGS和AGT进行免疫印迹时，结节病患者血清抗体谱差异很大。与AGS反应时，最常出现与79、27、30和50 kDa抗原决定簇的反应；与AGT反应时，则最常出现与17、35、32 kDa抗原决定簇的反应。
