Shinkareva T I, Kiroshka V S, Ginda S S, Gil'burd B Sh
Lab Delo. 1991(4):59-62.
Antibodies to three mycobacterial antigens, Soviet tuberculin and ultrasound-treated Mycobacterium tuberculosis (H37Rv) and M. bovis (BCG) were detected by enzyme immunoassay in 90 patients with pulmonary tuberculosis and 75 normal subjects. Antibody detection rates and levels were found related to the form of tuberculous process. The detection rate was rather low in focal tuberculosis and virtually the same for all the antigens (15.3 percent). In disseminated processes (infiltrative and particularly disseminated and fibrous-cavernous) antibodies are detected more often in higher titers than in local processes, and their levels are different with different antigens. The method was more sensitive with ultrasound-treated antigens of M. tuberculosis (H37RV) and M. bovis (BCG) than with PPD. Simultaneous enzyme immunoassay with three antigens detected up to 75 percent of antituberculous antibodies in infiltrative, up to 92 percent of antibodies in disseminated, and up to 100 percent of antibodies in fibrous-cavernous tuberculosis.
采用酶免疫测定法，在90例肺结核患者和75名正常受试者中检测了针对三种分枝杆菌抗原、苏联结核菌素以及经超声处理的结核分枝杆菌（H37Rv）和牛分枝杆菌（卡介苗）的抗体。发现抗体检测率和水平与结核病变的类型有关。在局灶性肺结核中，检测率相当低，且所有抗原的检测率几乎相同（15.3%）。在播散性病变（浸润性，尤其是播散性和纤维空洞性）中，与局部病变相比，抗体检测到的频率更高，滴度也更高，并且不同抗原的抗体水平有所不同。该方法对经超声处理的结核分枝杆菌（H37RV）和牛分枝杆菌（卡介苗）抗原比结核菌素纯蛋白衍生物（PPD）更敏感。同时用三种抗原进行酶免疫测定，在浸润性肺结核中可检测到高达75%的抗结核抗体，在播散性肺结核中可检测到高达92%的抗体，在纤维空洞性肺结核中可检测到高达100%的抗体。
