Sanchez J, Sarue A M, Brunet A, Monod H
Laboratoire de Physiologie du Travail-UA 385-CNRS, Faculté de Médecine, Pitié-Salpêtrière, Université Pierre et Marie Curie, Paris, France.
Arch Int Physiol Biochim Biophys. 1991 Feb;99(1):129-34. doi: 10.3109/13813459109145915.
The effects of training and naftidrofuryl treatment were observed in 21-month-old Long-Evans rats. Rats were injected intraperitoneally twice daily for 8 weeks with 7 mg.kg-1 of naftidrofuryl acid (SN, TN), or with 7 mg.kg-1 fumaric acid (SC and TC) or used as solvent. Training groups (TC, TN) started a progressive 8-week training programme of treadmill exercise. The activities of lactate dehydrogenase (LDH), hexokinase (HK), citrate synthase (CS) and 3-hydroxyacyl-Co-A-dehydrogenase (HAD), were measured in Soleus (SOL), Extensor Digitorum Longus (EDL) and Diaphragm (DIA) muscles. The mean VO2max value was 65 ml.min-1.kg-1 for 21-month-old rats. The training protocol induced increases in the mean VO2max values in the TC and TN groups, 71.8 and 74.4 ml.min-1.kg-1. In sedentary groups (SN), naftidrofuryl increased enzymatic activities (HK, CS, HAD) in the three muscles examined. When the animals underwent 8 weeks of physical training, the enzymatic activities (HK, CS, HAD) increased in SOL, EDL and DIA. When training was combined with naftidrofuryl treatment the increases in enzymatic activities were greater than those induced by training alone. However, the total changes did not differ for the sum of the changes produced by each condition alone.
在21月龄的Long-Evans大鼠中观察了训练和萘呋胺酯治疗的效果。大鼠每天腹腔注射两次，连续8周，注射7mg·kg-1的萘呋胺酸（SN，TN），或7mg·kg-1的富马酸（SC和TC），或用作溶剂。训练组（TC，TN）开始了为期8周的渐进式跑步机运动训练计划。在比目鱼肌（SOL）、趾长伸肌（EDL）和膈肌（DIA）中测量乳酸脱氢酶（LDH）、己糖激酶（HK）、柠檬酸合酶（CS）和3-羟酰基辅酶A脱氢酶（HAD）的活性。21月龄大鼠的平均最大摄氧量（VO2max）值为65ml·min-1·kg-1。训练方案使TC组和TN组的平均VO2max值增加，分别为71.8和74.4ml·min-1·kg-1。在久坐组（SN）中，萘呋胺酯增加了所检测的三块肌肉中的酶活性（HK、CS、HAD）。当动物进行8周的体育训练时，SOL、EDL和DIA中的酶活性（HK、CS、HAD）增加。当训练与萘呋胺酯治疗相结合时，酶活性的增加大于单独训练所诱导的增加。然而，每种条件单独产生的变化之和的总变化并无差异。
