Edwards Martin A, Martin Sophie, Whitworth Anna L, Macpherson Julie V, Unwin Patrick R
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
Physiol Meas. 2006 Dec;27(12):R63-108. doi: 10.1088/0967-3334/27/12/R01. Epub 2006 Oct 17.
This review highlights numerous and wide ranging biophysical and biochemical applications of scanning electrochemical microscopy (SECM). SECM instrumentation and theoretical modelling, necessary for experimental interpretation, are outlined, followed by a detailed discussion of the diverse applications of this technique. These include the measurement of flow through membranes, the determination of kinetic parameters of reactions, the investigation of the permeability of small molecules in tissues and monitoring biological processes, such as the production of oxygen or nitric oxide by cells. The significant impact of micro-electrochemical techniques on our understanding of basic physicochemical processes at biologically relevant interfaces is also considered. Studies reviewed include transport across and within bilayers and monolayers. Recent advances in SECM include the combination of SECM with other techniques, such as atomic force microscopy and optical microscopy. These developments are highlighted, along with prospects for the future.
本综述重点介绍了扫描电化学显微镜（SECM）在众多广泛的生物物理和生化方面的应用。概述了实验解释所需的SECM仪器和理论模型，随后详细讨论了该技术的各种应用。这些应用包括膜通量的测量、反应动力学参数的测定、小分子在组织中的渗透性研究以及生物过程的监测，如细胞产生氧气或一氧化氮。还考虑了微电化学技术对我们理解生物相关界面基本物理化学过程的重大影响。所综述的研究包括跨双层和单层以及在双层和单层内的传输。SECM的最新进展包括将SECM与其他技术（如原子力显微镜和光学显微镜）相结合。突出介绍了这些进展以及未来的前景。
