Kucia M, Halasa M, Wysoczynski M, Baskiewicz-Masiuk M, Moldenhawer S, Zuba-Surma E, Czajka R, Wojakowski W, Machalinski B, Ratajczak M Z
Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
Leukemia. 2007 Feb;21(2):297-303. doi: 10.1038/sj.leu.2404470. Epub 2006 Nov 30.
Recently, we purified from adult murine bone marrow (BM) a population of CXCR4(+), Oct-4(+) SSEA-1(+), Sca-1(+) lin(-) CD45(-) very small embryonic-like (VSEL) stem cells and hypothesized that similar cells could be also present in human cord blood (CB). Here, we report that by employing a novel two-step isolation procedure -- removal of erythrocytes by hypotonic lysis combined with multiparameter sorting -- we could isolate from CB a population of human cells that are similar to murine BM-derived VSELs, described previously by us. These CB-isolated VSELs (CB-VSEL) are very small (3-5 micro m) and highly enriched in a population of CXCR4(+)AC133(+)CD34(+)lin(-) CD45(-) CB mononuclear cells, possess large nuclei containing unorganized euchromatin and express nuclear embryonic transcription factors Oct-4 and Nanog and surface embryonic antigen SSEA-4. Further studies are needed to see if human CB-isolated VSELs similar to their murine BM-derived counterparts are endowed with pluripotent stem cell properties.
最近，我们从成年小鼠骨髓（BM）中纯化出一群CXCR4(+)、Oct-4(+)、SSEA-1(+)、Sca-1(+)、lin(-)、CD45(-)的非常小的胚胎样（VSEL）干细胞，并推测人类脐带血（CB）中也可能存在类似细胞。在此，我们报告，通过采用一种新颖的两步分离程序——低渗裂解去除红细胞并结合多参数分选——我们能够从脐带血中分离出一群与我们之前描述的源自小鼠骨髓的VSELs相似的人类细胞。这些从脐带血中分离出的VSELs（CB-VSEL）非常小（3-5微米），高度富集于CXCR4(+)、AC133(+)、CD34(+)、lin(-)、CD45(-)的脐带血单个核细胞群体中，具有含有无组织常染色质的大细胞核，并表达核胚胎转录因子Oct-4和Nanog以及表面胚胎抗原SSEA-4。还需要进一步研究，以确定与源自小鼠骨髓的对应细胞相似的从人类脐带血中分离出的VSELs是否具有多能干细胞特性。
