Reid M S, Herrera-Marschitz M, Ungerstedt U
Department of Pharmacology, Karolinska Institute, Stockholm, Sweden.
J Neurochem. 1991 Sep;57(3):970-4. doi: 10.1111/j.1471-4159.1991.tb08245.x.
Extracellular levels of dopamine (DA) and its metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), in the striatum and frontoparietal (sensorimotor) cortex in halothane-anesthetized rats were analyzed simultaneously using in vivo microdialysis. Basal DA levels, measured from the microdialysis perfusate, were 6.4 +/- 0.8 nM (n = 15) in the striatum and 0.9 +/- 0.1 nM (n = 15) in the frontoparietal cortex. Subcutaneous injections of d-amphetamine (2 mg/kg) increased DA levels 10-fold in the striatum and fivefold in the cortex. Injections of substance P (0.07 nmol/0.2 microliters) into the substantia nigra pars reticulata (SNR) increased DA and DOPAC levels approximately 30% in the ipsilateral striatum and approximately 50% in the ipsilateral frontoparietal cortex. Injections of neurokinin A (0.09 nmol/0.2 microliter) into the SNR increased DA and DOPAC levels approximately 30% in the ipsilateral striatum but did not significantly affect DA levels in the ipsilateral frontoparietal cortex, although DOPAC levels were increased by approximately 50%. It is suggested that striatal and cortical DA release is regulated differently by nigral substance P and neurokinin A terminals.
使用体内微透析技术，同时分析了氟烷麻醉大鼠纹状体和额顶叶（感觉运动）皮质中多巴胺（DA）及其代谢产物3,4-二羟基苯乙酸（DOPAC）的细胞外水平。从微透析灌流液中测得的基础DA水平，在纹状体中为6.4±0.8 nM（n = 15），在额顶叶皮质中为0.9±0.1 nM（n = 15）。皮下注射d-苯丙胺（2 mg/kg）可使纹状体中的DA水平增加10倍，使皮质中的DA水平增加5倍。向黑质网状部（SNR）注射P物质（0.07 nmol/0.2微升）可使同侧纹状体中的DA和DOPAC水平增加约30%，使同侧额顶叶皮质中的DA和DOPAC水平增加约50%。向SNR注射神经激肽A（0.09 nmol/0.2微升）可使同侧纹状体中的DA和DOPAC水平增加约30%，但对同侧额顶叶皮质中的DA水平无显著影响，尽管DOPAC水平增加了约50%。提示黑质P物质和神经激肽A终末对纹状体和皮质DA释放的调节方式不同。
