Baroukh B, Saffar J L
Département de Parodontologie, Faculté de Chirurgie Dentaire, Université Paris-V, France.
J Periodontal Res. 1991 May;26(3 Pt 1):161-6. doi: 10.1111/j.1600-0765.1991.tb01640.x.
Quantification of osteoclast resorption, a good index of periodontitis destruction, is primarily based on osteoclast identification. As their identification is sometimes dubious, we compared osteoclastic counts in hamster specimens processed for either routine histology or tartrate-resistant acid phosphatase (TRAP) staining. No difference was found between the two approaches concerning the number of osteoclasts. However the mean bone-osteoclast interface was higher in the TRAP-stained specimens (+30%, p less than 0.02). As osteoclast precursors are also TRAP+ cells, they were quantified too. Compared with controls, there was a dramatic increase (p less than 0.0001) in periodontitis-affected animals. Precursors were strongly correlated to active osteoclasts (r = 0.97). Our data suggest that precursors are recruited only when the disease is active in a given site.
破骨细胞吸收的量化是牙周炎破坏的一个良好指标，主要基于破骨细胞的识别。由于它们的识别有时存在疑问，我们比较了用常规组织学或抗酒石酸酸性磷酸酶（TRAP）染色处理的仓鼠标本中的破骨细胞计数。两种方法在破骨细胞数量方面没有差异。然而，TRAP染色标本中的平均骨-破骨细胞界面更高（增加30%，p小于0.02）。由于破骨细胞前体也是TRAP+细胞，因此也对它们进行了量化。与对照组相比，牙周炎感染动物中有显著增加（p小于0.0001）。前体与活跃破骨细胞密切相关（r = 0.97）。我们的数据表明，仅当疾病在特定部位活跃时才会招募前体。
