D'Annibale Alessandro, Leonardi Vanessa, Federici Ermanno, Baldi Franco, Zecchini Fulvio, Petruccioli Maurizio
Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via S. C. De Lellis, 01100 Viterbo, Italy.
Appl Microbiol Biotechnol. 2007 Apr;74(5):1135-44. doi: 10.1007/s00253-006-0749-z. Epub 2006 Nov 29.
Contaminated soil from a historical industrial site and containing sulfide ore ashes and aromatic hydrocarbons underwent sequential leaching by 0.5 M citrate and microbial treatments. Heavy metals leaching was with the following efficiency scale: Cu (58.7%) > Pb (55.1%) > Zn (44.5%) > Cd (42.9%) > Cr (26.4%) > Ni (17.7%) > Co (14.0%) > As (12.4%) > Fe (5.3%) > Hg (1.1%) and was accompanied by concomitant removal of organic contaminants (about 13%). Leached metals were concentrated into an iron gel, produced during ferric citrate fermentation by the metal-resistant strain BAS-10 of Klebsiella oxytoca. Concomitantly, the acidic leached soil was bioaugmented with Allescheriella sp. DABAC 1, Stachybotrys sp. DABAC 3, Phlebia sp. DABAC 9, Pleurotus pulmonarius CBS 664.97, and Botryosphaeria rhodina DABAC P82. B. rhodina was most effective, leading to a significant depletion of the most abundant contaminants, including 7-H-benz[DE]anthracene-7-one, 9,10-anthracene dione and dichloroaniline isomers, and to a marked detoxification as assessed by the mortality test with the Collembola Folsomia candida Willem. The overall degradation activities of B. rhodina and P. pulmonarius appeared to be significantly enhanced by the preliminary metal removal.
来自一个历史工业场地、含有硫化矿灰和芳烃的污染土壤，先后用0.5M柠檬酸盐进行淋洗并进行微生物处理。重金属的淋洗效率顺序如下：铜（58.7%）>铅（55.1%）>锌（44.5%）>镉（42.9%）>铬（26.4%）>镍（17.7%）>钴（14.0%）>砷（12.4%）>铁（5.3%）>汞（1.1%），同时伴随有机污染物的去除（约13%）。淋出的金属被浓缩到一种铁凝胶中，该铁凝胶是由产酸克雷伯菌的耐金属菌株BAS-10在柠檬酸铁发酵过程中产生的。同时，酸性淋洗后的土壤用阿利氏菌属DABAC 1、葡萄穗霉属DABAC 3、脉菌属DABAC 9、肺形侧耳CBS 664.97和葡萄座腔菌DABAC P82进行生物强化。葡萄座腔菌最为有效，导致包括7-H-苯并[DE]蒽-7-酮、9,10-蒽二酮和二氯苯胺异构体在内的最丰富污染物显著减少，并通过用白符跳（Folsomia candida Willem）进行的死亡率测试评估显示出明显的解毒作用。葡萄座腔菌和肺形侧耳的总体降解活性似乎因初步的金属去除而显著增强。
