Grower M F, Ficara A J, Chandler D W, Kramer G D
J Periodontol. 1975 Nov;46(11):669-72. doi: 10.1902/jop.1975.46.11.669.
Gingival fluid from eight control subjects and ten diabetics was collected and the content of cAMP and protein in the fluid was assayed to determine the nature of the biochemical changes occurring in the gingival fluid due to diabetes. The gingival fluid of the control subjects had a cAMP concentration of 2.4 X 10(-6) M, which was a hundredfold greater than that seen in serum, thus suggesting that the cAMP in the fluid resulted from active synthesis by the gingival cells and was not merely a transudate from the blood. The gingival fluid of the diabetics contained only one-seventh the level of cAMP seen in the control group. It is suggested that the decreased level of cAMP seen in the givgival fluid of diabetics may be a manifestation of a defect in the cAMP forming mechanism of the gingival tissue, which may reflect the systemic etiology of diabetes. It was also found that in the control subjects the content of cAMP in the gingival fluid was in inverse proportion to the volume of exudate in the gingival crevice. No such relationship was seen in the diabetic group. It appears that the level of cAMP present in the gingival fluid of normal individuals without any generalized endocrine deficiencies may be used as an additional indicator of the inflammatory status of the gingival tissues, along with the clinical evaluation based on gingival index.
收集了8名对照受试者和10名糖尿病患者的龈沟液，并检测了龈沟液中cAMP和蛋白质的含量，以确定糖尿病导致的龈沟液中发生的生化变化的性质。对照受试者的龈沟液中cAMP浓度为2.4×10（-6）M，比血清中高出100倍，这表明龈沟液中的cAMP是由牙龈细胞主动合成产生的，而不仅仅是血液的渗出液。糖尿病患者的龈沟液中cAMP水平仅为对照组的七分之一。有人认为，糖尿病患者龈沟液中cAMP水平降低可能是牙龈组织cAMP形成机制缺陷的一种表现，这可能反映了糖尿病的全身病因。还发现，对照受试者龈沟液中cAMP的含量与龈沟渗出液的量成反比。糖尿病组未观察到这种关系。看来，在没有任何全身性内分泌缺陷的正常个体的龈沟液中，cAMP水平可作为牙龈组织炎症状态的额外指标，同时结合基于牙龈指数的临床评估。
