用对李假单胞菌的O抗血清检测铜绿假单胞菌的常见多糖抗原。

Detection of common polysaccharide antigen of Pseudomonas aeruginosa with O-antiserum to Pseudomonas cerasi.

作者信息

Kocharova N A, Knirel Y A, Romanowska A, Romanowska E, Makarenko T A, Edvabnaya L S, Stanislavsky E S, Zdorovenko G M, Yakovleva L M

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow.

出版信息

FEMS Microbiol Immunol. 1991 Apr;3(2):69-73. doi: 10.1111/j.1574-6968.1991.tb04199.x.

DOI:10.1111/j.1574-6968.1991.tb04199.x

PMID:1713775

Abstract

The identity of the structures of common polysaccharide antigen (CPA) of Pseudomonas aeruginosa and O-antigen of Pseudomonas cerasi was used for immunochemical study of polysaccharide antigens of seven immunotypes (IT) of P. aeruginosa. ELISA performed with O-antiserum to P. cerasi showed that CPA is present in all seven ITs in different amounts. In SDS-PAGE this antigen behaves as a lipopolysaccharide (LPS) and is detected by immunoblotting technique in five of seven ITs.

摘要

利用铜绿假单胞菌常见多糖抗原（CPA）与樱桃假单胞菌O抗原结构的一致性，对铜绿假单胞菌7种免疫型（IT）的多糖抗原进行免疫化学研究。用针对樱桃假单胞菌的O抗血清进行的ELISA检测表明，所有7种免疫型中均存在不同量的CPA。在SDS-PAGE中，该抗原表现为脂多糖（LPS），通过免疫印迹技术在7种免疫型中的5种中检测到。