Schoenmakers H C, Koornneef M, Alefs S J, Gerrits W F, van der Kop D, Chérel I, Caboche M
Dept. of Genetics, Agricultural University Wageningen, The Netherlands.
Mol Gen Genet. 1991 Jul;227(3):458-64. doi: 10.1007/BF00273938.
Five nitrate reductase-deficient mutants of tomato were isolated from an M2 population after ethylmethanesulphonate (EMS) seed treatment by means of selection for chlorate resistance. All mutations were monogenic and recessive and complementation analysis revealed that they were non-allelic. Biochemical and molecular characterization of these mutants showed that four of them are cofactor mutants while one is an apoenzyme mutant.
通过对氯酸盐抗性的筛选,从经甲基磺酸乙酯(EMS)处理的番茄种子M2群体中分离出五个硝酸盐还原酶缺陷型突变体。所有突变均为单基因且隐性,互补分析表明它们是非等位基因。对这些突变体的生化和分子特征分析表明,其中四个是辅因子突变体,一个是脱辅基酶突变体。