Ong W Y, Garey L J
Laboratory of Neurobiology, National Institute for Medical Research, London, UK.
Anat Embryol (Berl). 1991;183(4):397-413. doi: 10.1007/BF00196841.
Antibodies were used to identify neurons in human frontal and temporal cortex that were immuno-positive to gamma-aminobutyric acid (GABA) and the neuropeptides vasoactive intestinal polypeptide (VIP), substance P (SP) and somatostatin (SOM). Specimens were taken at surgical biopsy and fixed immediately after removal. The results described for both light and electron microscopy were obtained when relatively high concentrations of glutaraldehyde (2.5-3%) were present in the fixative. Specimens were examined from three adults and an infant aged 5 months. GABAergic neurons were present in all cortical layers, with fewest in layers I, deep III and V, and were mainly small, and round or oval. No labelled pyramidal neurons were detected. GABAergic puncta were common in the neuropil, probably representing axonal profiles. VIP-neurons were also found in all layers, including layer I, and were approximately twice as numerous as GABA-cells. SP-positive cells were found throughout the layers, but were sparse in layers I and VI. They were about three times commoner than GABAergic neurons. SOM-reactivity was demonstrated in about the same number of cells as that for SP. Again, this involved all layers, but layer I least. Peptidergic neurons were larger, on the average, than GABAergic cells, and were frequently pyramidal in character. In the infant, the distribution, size and frequency of immunoreactive neurons were similar to those in the adult. However, GABAergic puncta were commoner.
抗体用于鉴定人类额叶和颞叶皮质中对γ-氨基丁酸（GABA）以及神经肽血管活性肠多肽（VIP）、P物质（SP）和生长抑素（SOM）呈免疫阳性的神经元。标本取自手术活检，取出后立即固定。当固定液中存在相对高浓度的戊二醛（2.5 - 3%）时，获得了光镜和电镜描述的结果。对三名成年人和一名5个月大的婴儿的标本进行了检查。GABA能神经元存在于所有皮质层，在I层、深层III和V层中数量最少，主要为小型、圆形或椭圆形。未检测到标记的锥体神经元。GABA能小点在神经毡中很常见，可能代表轴突轮廓。VIP神经元在所有层中也有发现，包括I层，其数量约为GABA细胞的两倍。SP阳性细胞在各层均有发现，但在I层和VI层中稀疏。它们比GABA能神经元大约多两倍。SOM反应性在与SP相同数量的细胞中得到证实。同样，这涉及所有层，但I层最少。肽能神经元平均比GABA能细胞大，且通常呈锥体特征。在婴儿中，免疫反应性神经元的分布、大小和频率与成年人相似。然而，GABA能小点更为常见。
