Nespeca Gilles, Grest Paula, Rosenkrantz Wayne S, Ackermann Mathias, Favrot Claude
Clinic for Small Animal Internal Medicine, Dermatology Unit, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland.
Am J Vet Res. 2006 Dec;67(12):2036-41. doi: 10.2460/ajvr.67.12.2036.
To detect and partially characterize papillomavirus (PV) DNA in squamous cell carcinoma (SCC) tumor specimens from cats.
54 formalin-fixed paraffinembedded skin biopsy specimens were examined. Specimens originated from Bowenoid in situ SCC (BISC; n = 21), invasive SCC (22), and skin affected by miscellaneous nonneoplastic conditions (11).
Samples from each tissue block underwent DNA extraction after deparaffinization, and PCR assays were performed. Two sets of primers derived from PV E1 were used. The first set of primers was designed for the narrow-range PCR assay and was able to generate amplification products of feline PV (FePV), canine oral PV, or closely related PVs. The second set of primers was selected for the broad-range PCR assay because of its ability to amplify DNA from 64 human PVs. Sequence analysis of each amplified DNA was performed.
1 of the 21 specimens of BISC was positive for PV DNA on the basis of narrow-range PCR assay results, whereas all the other specimens (BISC, invasive SCC, and controls) had negative results for PV DNA. In contrast, 5 of 21 BISC specimens and 4 of 22 invasive SCC specimens were positive for PV DNA on the basis of broad-range PCR assay results. Sequence analysis revealed that only 1 specimen was infected by a virus closely related to classic FePV. In the 8 other specimens positive for PV DNA, DNA of unknown PVs was uncovered.
Bowenoid in situ SCC and invasive SCC of cats may be associated with PVs of genetic diversity.
检测猫鳞状细胞癌（SCC）肿瘤标本中的乳头瘤病毒（PV）DNA并对其进行部分特征分析。
对54份福尔马林固定石蜡包埋的皮肤活检标本进行检查。标本来源于原位鲍恩样鳞状细胞癌（BISC；n = 21）、浸润性SCC（22份）以及受其他非肿瘤性疾病影响的皮肤（11份）。
每个组织块的样本经脱石蜡处理后进行DNA提取，并进行聚合酶链反应（PCR）检测。使用了两组源自PV E1的引物。第一组引物用于窄范围PCR检测，能够扩增猫乳头瘤病毒（FePV）、犬口腔乳头瘤病毒或密切相关的乳头瘤病毒的扩增产物。第二组引物用于宽范围PCR检测，因其能够扩增64种人乳头瘤病毒的DNA。对每个扩增的DNA进行序列分析。
根据窄范围PCR检测结果，21份BISC标本中有1份PV DNA呈阳性，而所有其他标本（BISC、浸润性SCC和对照）的PV DNA检测结果均为阴性。相比之下，根据宽范围PCR检测结果，21份BISC标本中有5份PV DNA呈阳性，22份浸润性SCC标本中有4份PV DNA呈阳性。序列分析显示，只有1份标本感染了与经典FePV密切相关的病毒。在其他8份PV DNA呈阳性的标本中，发现了未知乳头瘤病毒的DNA。
猫的原位鲍恩样鳞状细胞癌和浸润性鳞状细胞癌可能与具有遗传多样性的乳头瘤病毒有关。
