Wilson K H, Blitchington R, Frothingham R, Wilson J A
Division of Infectious Diseases, Duke University Medical Center, Durham, North Carolina.
Lancet. 1991 Aug 24;338(8765):474-5. doi: 10.1016/0140-6736(91)90545-z.
Efforts to culture and identify the intracellular bacteria associated with Whipple's disease have been unsuccessful. Nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 S ribosomal DNA present in a small-bowel biopsy specimen taken from a patient with Whipple's disease. A search by computer for similar rRNA sequences filed in databases showed the Whipple's-associated organism to be most similar to bacteria of the Rhodococcus, Streptomyces, and Arthrobacter genera, and more weakly related to mycobacteria. The biopsy specimen was estimated to contain around 10(7) cells of the organism. The probable aetiological agent for our patient's illness has not been identified previously in a patient with Whipple's disease.
培养和鉴定与惠普尔病相关的细胞内细菌的努力一直未成功。对取自一名惠普尔病患者的小肠活检标本中存在的细菌16S核糖体DNA进行了核苷酸测序和聚合酶链反应扩增。通过计算机搜索数据库中存档的相似rRNA序列，发现与惠普尔病相关的生物体与红球菌属、链霉菌属和节杆菌属的细菌最为相似，与分枝杆菌的关系较弱。估计活检标本中含有约10⁷个该生物体的细胞。我们患者疾病的可能病原体此前在惠普尔病患者中尚未被鉴定出来。
