Isakova A V, Mednikova Iu S
Zh Vyssh Nerv Deiat Im I P Pavlova. 2006 Sep-Oct;56(5):664-73.
The effects of acetylcholine and noradrenaline applications on neuronal sponta-neous activity were investigated in slices of guinea-pig parietal cortex. Iontophoretic ejections of both neurotransmitters to the cortical neurons evoked the same-type slowly-developing and long-lasting increase in the rate of spike activity. The different temperature sensitivity of cholinergic and noradrenergic reactions were revealed. During the temperature shift from 32-34 degrees C to 35-36 degrees C the cholinergic effect on neuronal spike activity became extremely strong, that is why even silent at t = 32-32 degrees C neurons became to acetylcholine responsive. Temperature-dependent changes in spike reaction to acetylcholine were accompanied by stable increase in spontaneous spike activity. The noradrenergic reactions did not change with temperature in limits from 32-34 to 35-36 degrees C. In this temperature range spike reactions to glutamate, the main excitation transmitter in the cortex, remained constant. The results obtained suggest that acetylcholine is the main neurotransmitter regulating spontaneous spike activity in cortical neurons.
在豚鼠顶叶皮层切片中研究了乙酰胆碱和去甲肾上腺素应用对神经元自发活动的影响。通过离子电泳将两种神经递质喷射到皮层神经元上，均引起相同类型的、缓慢发展且持久的放电活动速率增加。揭示了胆碱能和去甲肾上腺素能反应的不同温度敏感性。在温度从32 - 34摄氏度转变为35 - 36摄氏度的过程中，胆碱能对神经元放电活动的影响变得极其强烈，这就是为什么在t = 32 - 32摄氏度时沉默的神经元变得对乙酰胆碱有反应。对乙酰胆碱的放电反应中与温度相关的变化伴随着自发放电活动的稳定增加。在32 - 34摄氏度至35 - 36摄氏度的范围内，去甲肾上腺素能反应不随温度变化。在这个温度范围内，对皮层中主要的兴奋性递质谷氨酸的放电反应保持恒定。所获得的结果表明，乙酰胆碱是调节皮层神经元自发放电活动的主要神经递质。
