Mednikova Iu S, Loseva E V, Karnup S V, Zhadin M N
Zh Vyssh Nerv Deiat Im I P Pavlova. 1996 Sep-Oct;46(5):893-903.
In slices of parietal cortex of a guinea pig spike reactions were studied induced in neurons by iontophoretical applications of acetylcholine to their somata and dendrites. The results were obtained from 128 units. When applied to different sites of the neuronal membrane acetylcholine produced an increase in firing activity nearly in the same percent of cases (50-75%). The reactions to acetylcholine were most typical for spontaneously active neurons. The slow onset (to 8 sec) and long duration (to 25 sec) of responses evoked by acetylcholine point to an involvement of muscarinic receptors. Spike responses evoked by acetylcholine application to soma and dendrites were of the same latencies and magnitude. The most distant dendritic site where the acetylcholine excitation was able to evoke response of the soma was separated from it by 300-400 mcm. It is suggested that acetylcholine excitation propagates from dendritic points to the soma with intracellular biochemical processes.
在豚鼠顶叶皮层切片中，研究了通过向神经元胞体和树突进行离子电渗法施加乙酰胆碱所诱导的神经元锋电位反应。结果来自128个单位。当乙酰胆碱应用于神经元膜的不同部位时，在几乎相同比例的情况（50 - 75%）下会使放电活动增加。对乙酰胆碱的反应在自发活动的神经元中最为典型。乙酰胆碱诱发的反应起效缓慢（长达8秒）且持续时间长（长达25秒），这表明毒蕈碱受体参与其中。向胞体和树突施加乙酰胆碱所诱发的锋电位反应具有相同的潜伏期和幅度。乙酰胆碱兴奋能够诱发胞体反应的最远树突部位与胞体相距300 - 400微米。有人提出，乙酰胆碱兴奋通过细胞内生化过程从树突部位传播到胞体。
