抗莱氏无胆甾原体膜Mg(2+)-ATP酶抗体的免疫学特性
Immunological properties of antibodies against Mg(2+)-ATPase from Acholeplasma laidlawii membranes.
作者信息
Chen J W, Zhang L P, Hwang F
机构信息
Department of Biomembranes, Institute of Biophysics, Academia Sinica, Beijing, China.
出版信息
Biochem Int. 1991 Mar;23(4):791-9.
In the present study, antibodies were raised against the Mg(2+)-ATPase and the immunological relationships between the enzyme and other ATPase from a variety of biological membranes were determined. The anti Mg(2+)-ATPase antiserum inhibited 85% of the enzyme activity from A. laidlawii membranes. We demonstrate a specific selectivity of Mg(2+)-ATPase antiserum for antigenic determinants of the A. laidlawii membranes. Immunoblot studies of A. laidlawii membrane peptides indicated labeling of five bands, 66KD, 49KD, 34KD, 26KD and 13KD, corresponding to five subunits of the ATPase in A. laidlawii membranes.
在本研究中，制备了针对Mg(2+)-ATP酶的抗体，并测定了该酶与来自各种生物膜的其他ATP酶之间的免疫关系。抗Mg(2+)-ATP酶抗血清抑制了莱氏无胆甾原体膜中85%的酶活性。我们证明了Mg(2+)-ATP酶抗血清对莱氏无胆甾原体膜抗原决定簇具有特异性选择性。莱氏无胆甾原体膜肽的免疫印迹研究表明，有五条带被标记，分别为66KD、49KD、34KD、26KD和13KD，对应于莱氏无胆甾原体膜中ATP酶的五个亚基。
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