Vinther O
J Gen Microbiol. 1980 Feb;116(2):451-7. doi: 10.1099/00221287-116-2-451.
The ultrastructure of agar-grown Acholeplasma laidlawii incubated with specific antiserum or IgM fractions of this antiserum has been investigated by the thin-sectioning technique. Antiserum treatment resulted in the development of giant cells along the colony circumferences and in the coating of normal-size mycoplasmas with a periodically arranged extramembranous layer, consisting of attached immunoglobulins as shown by indirect immunoferritin labelling. The regular structure of the coat was not influenced by changes in temperature or by fixation of the membrane antigens prior to reaction with antibody. Extracellular enveloped viruses were uniformly covered with antibody in these experiments. IgM fractions of antiserum in high concentrations produced a similarly uniform extramembranous layer both on mycoplasmas and viruses. Possible explanations of the difference demonstrated between the regular arrangement of antigenic determinants on A. laidlawii membranes and the previously observed uniform binding of immunoglobulins to Mycoplasma gallisepticum are discussed.
运用超薄切片技术，对与特异性抗血清或该抗血清的IgM组分一起培养的琼脂培养的莱氏无胆甾原体的超微结构进行了研究。抗血清处理导致菌落周边出现巨细胞，并使正常大小的支原体被一层周期性排列的膜外层所覆盖，间接免疫铁蛋白标记显示该膜外层由附着的免疫球蛋白组成。在与抗体反应之前，温度变化或膜抗原固定均不影响被膜的规则结构。在这些实验中，细胞外包膜病毒被抗体均匀覆盖。高浓度抗血清的IgM组分在支原体和病毒上均产生了类似的均匀膜外层。文中讨论了莱氏无胆甾原体膜上抗原决定簇的规则排列与先前观察到的免疫球蛋白与鸡毒支原体的均匀结合之间差异的可能解释。
