补体介导的针对莱氏无胆甾原体各种膜成分的抗体杀伤作用。
Complement-mediated killing of Acholeplasma laidlawii by antibodies to various membrane components.
作者信息
Dörner I, Brunner H, Schiefer H G, Wellensiek H J
出版信息
Infect Immun. 1976 Jun;13(6):1663-70. doi: 10.1128/iai.13.6.1663-1670.1976.
Mycoplasmas are useful models for biochemical studies of the mechanism of complement-mediated killing by antibodies to various membrane components. The purpose of this study was to determine the membrane antigens involved in immune killing of Acholeplasma laidlawii. Antibodies to A. laidlawii membrane total lipids, glycolipids, and phospholipids could be induced in rabbits after injection of reaggregates of the purified lipids with Mycoplasma hominis protein as the carrier. Killing of A. laidlawii membrane lipids were less effective than anti-membrane protein antisera in killing the organisms. Of the antisera to lipid components of A. laidlawii membranes, antiserum to phospholipids showed a more pronounced killing effect than antiserum to glycolipids. The antibodies to A. laidlawii in the rabbit antisera belong predominantly to the immunoglobulin G class of immunoglobulins. Double-diffusion tests in agar indicated that two immunologically reactive proteins are located on the membrane surface.
支原体是研究抗体针对各种膜成分通过补体介导杀伤机制的生化过程的有用模型。本研究的目的是确定参与莱氏无胆甾原体免疫杀伤的膜抗原。以人型支原体蛋白为载体，将纯化脂质的再聚集物注射到兔体内后，可诱导产生针对莱氏无胆甾原体膜总脂质、糖脂和磷脂的抗体。在杀伤莱氏无胆甾原体方面，抗膜脂质抗体不如抗膜蛋白抗血清有效。在针对莱氏无胆甾原体膜脂质成分的抗血清中，抗磷脂抗血清的杀伤作用比抗糖脂抗血清更明显。兔抗血清中针对莱氏无胆甾原体的抗体主要属于免疫球蛋白G类。琼脂双向扩散试验表明，膜表面有两种免疫反应性蛋白。
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