Grossmann Tom N, Seitz Oliver
Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, D-12489 Berlin, Germany.
J Am Chem Soc. 2006 Dec 13;128(49):15596-7. doi: 10.1021/ja0670097.
Herein we describe a novel DNA-catalyzed transfer of a reporter group from a donating to an accepting oligo-peptide nucleic acid (PNA), generating high catalytic turnover numbers in a DNA-sequence specific manner. The demonstrated transfer of the Dabcyl group was designed to switch on emission of a fluorescein dye while switching off emission of a rhodamine dye. This setup allows the highly sensitive and selective detection of DNA-sequences in real time.
在此，我们描述了一种新型的DNA催化的报告基团从供体寡聚肽核酸（PNA）转移到受体PNA的过程，以DNA序列特异性方式产生高催化周转数。所展示的Dabcyl基团转移旨在开启荧光素染料的发射，同时关闭罗丹明染料的发射。这种设置允许实时对DNA序列进行高灵敏度和高选择性的检测。
