Cevenini R, Donati M, Brocchi E, De Simone F, La Placa M
Instituto di Microbiologia, University of Bologna, Italy.
FEMS Microbiol Lett. 1991 Jun 1;65(1):111-5. doi: 10.1016/0378-1097(91)90481-o.
An 89-kDa immunogen from Chlamydia psittaci A/22 causing ovine abortion was partially characterized. The 89-kDa protein, localized on the outer membrane complex of chlamydiae, was synthesized relatively early in the developmental cycle. The protein contained cysteine but was not extensively cross-linked by disulfide bonds. Treatment with proteases apparently did not cleave the protein. The infectivity of strain A/22 was partially (60%) reduced by treatment of chlamydial elementary bodies with monoclonal antibody BS/89 specifically reacting with the 89-kDa antigen. Species-specific as well as strain-specific antigenic determinants were present on the 89-kDa protein.
对引起绵羊流产的鹦鹉热衣原体A/22株的一种89千道尔顿免疫原进行了部分特性鉴定。这种89千道尔顿的蛋白质定位于衣原体的外膜复合物上，在发育周期中合成相对较早。该蛋白质含有半胱氨酸，但未被二硫键广泛交联。用蛋白酶处理显然不会切割该蛋白质。用与89千道尔顿抗原特异性反应的单克隆抗体BS/89处理衣原体原体，可使A/22株的感染性部分（60%）降低。89千道尔顿的蛋白质上存在种特异性以及菌株特异性抗原决定簇。
