Kobayashi N, Taniguchi K, Urasawa T, Urasawa S
Department of Hygiene, Sapporo Medical College, Chuo-ku, Japan.
J Gen Virol. 1991 Aug;72 ( Pt 8):1855-61. doi: 10.1099/0022-1317-72-8-1855.
Three anti-VP7 monoclonal antibodies (MAbs) which neutralized only two strains (K8 and S12) of five serotype 1 human rotaviruses (HRVs) were obtained, and neutralization epitopes recognized by these 'monotype-specific' MAbs were analysed by epitope mapping and sequencing of the VP7 genes. Neutralization-resistant mutants of K8 and S12 were selected by the monotype-specific MAbs and serotype 1-specific MAbs prepared previously. Cross-neutralization tests between MAbs and neutralization-resistant mutants of K8 and S12 indicated that epitopes of monotype-specific MAbs operationally overlap with those of serotype 1-specific and cross-reactive MAbs recognizing the S1 region. Sequence analyses of the VP7 genes indicated that VP7s of strains K8 and S12, which belong to a monotype of serotype 1 viruses, possessed amino acids at positions 42 and 87 different from other serotype 1 HRVs. Furthermore, amino acid substitution sites of representative mutants of K8, selected by the monotype-specific MAbs, were identified at positions 96, 97 and 100. These results imply that amino acids in variable region B (amino acids 87 to 101) are involved in the monotype-specific neutralization epitope as well as serotype-specific neutralization epitopes.
获得了三种仅能中和五株1型人类轮状病毒（HRV）中的两株（K8和S12）的抗VP7单克隆抗体（MAb），并通过表位作图和VP7基因测序分析了这些“单型特异性”MAb识别的中和表位。通过单型特异性MAb和先前制备的1型特异性MAb筛选出K8和S12的中和抗性突变体。MAb与K8和S12的中和抗性突变体之间的交叉中和试验表明，单型特异性MAb的表位与识别S1区域的1型特异性和交叉反应性MAb的表位在功能上重叠。VP7基因的序列分析表明，属于1型病毒单型的K8和S12株的VP7在第42和87位的氨基酸与其他1型HRV不同。此外，通过单型特异性MAb筛选出的K8代表性突变体的氨基酸取代位点位于第96、97和100位。这些结果表明，可变区B（第87至101位氨基酸）中的氨基酸既参与单型特异性中和表位，也参与血清型特异性中和表位。
