Ogawa S, Dohi K, Yamada H, Hirayama T, Morita H, Iwano M, Ishikawa H
First Department of Internal Medicine, Nara Medical University.
Nihon Jinzo Gakkai Shi. 1991 Apr;33(4):349-56.
In order to clarify the mechanism for elevation of serum IgE level in minimal change nephrotic syndrome (MCNS), we investigated Fc epsilon receptor 2 (Fc epsilon R2/CD23) expression, using fluorescein isothiocyanate (FITC) labeled anti-CD20 monoclonal antibody, phycoerythrin (PE) labeled anti-CD23 monoclonal antibody and two-color flow cytometry. Moreover, serum IgE level was examined by radio-immunosorbent test. The subjects included 25 cases of MCNS, 17 cases of focal glomerular sclerosis (FGS), and 25 healthy volunteers as controls. The patients in the nephrotic stage of MCNS demonstrated significantly elevated levels of serum IgE, while those in the remission stage of MCNS showed no change in their serum IgE levels. In patients with nephrotics of MCNS, CD23+ cells, CD20+ CD23+ cells and CD23/CD20 ratio were significantly increased compared to normal controls. Furthermore, CD23/CD20 ratio in MCNS was significantly correlated with serum IgE level. Concerning FGS, there were no differences in serum IgE level and Fc epsilon R2 expression compared to those of normal controls. These results suggest that Fc epsilon R2 expression may be important in the mechanism of elevated serum IgE level in MCNS.
为阐明微小病变肾病（MCNS）患者血清IgE水平升高的机制，我们采用异硫氰酸荧光素（FITC）标记的抗CD20单克隆抗体、藻红蛋白（PE）标记的抗CD23单克隆抗体及双色流式细胞术，对Fcε受体2（FcεR2/CD23）表达进行了研究。此外，通过放射免疫吸附试验检测血清IgE水平。研究对象包括25例MCNS患者、17例局灶性肾小球硬化（FGS）患者及25名健康志愿者作为对照。MCNS肾病期患者血清IgE水平显著升高，而MCNS缓解期患者血清IgE水平无变化。与正常对照相比，MCNS肾病患者的CD23+细胞、CD20+CD23+细胞及CD23/CD20比值显著增加。此外，MCNS患者的CD23/CD20比值与血清IgE水平显著相关。关于FGS，与正常对照相比，其血清IgE水平及FcεR2表达无差异。这些结果提示，FcεR2表达可能在MCNS患者血清IgE水平升高的机制中起重要作用。
