Carpentier Sebastien C, Witters Erwin, Laukens Kris, Van Onckelen Harry, Swennen Rony, Panis Bart
Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, Department of Biosystems, Katholieke Universiteit Leuven, Leuven, Belgium.
Proteomics. 2007 Jan;7(1):92-105. doi: 10.1002/pmic.200600533.
Banana (Musa spp.) multiple shoot meristems are an excellent model to study the meristem proteome. Using a 2-DE protocol developed for small amounts of tissue and MS-based cross species polypeptide identification, we have revealed the meristem proteome and investigated the influence of sucrose-mediated osmotic stress in a dehydration-tolerant variety. Proteins that were significantly up- or down-regulated due to the high-sucrose treatment were classified using non-parametric univariate statistics. Our results suggest that the maintenance of an osmoprotective intracellular sucrose concentration, the enhanced expression of particular genes of the energy-conserving glycolysis and the conservation of the cell wall integrity are essential to maintain homeostasis, to acclimate and to survive dehydration. By comparing the dehydration-tolerant variety with a dehydration-sensitive variety, we were able to distinguish several genotype-specific proteins (isoforms), and could associate the dehydration-tolerant variety with proteins involved in energy metabolism (e.g., phosphoglycerate kinase, phosphoglucomutase, UDP-glucose pyrophosphorylase) and proteins that are associated with stress adaptation (e.g., OSR40-like protein, abscisic stress ripening protein-like protein). This work shows that proteome analysis can be used successfully to perform quantitative difference analysis and to characterize genetic variations in a recalcitrant crop.
香蕉（芭蕉属）的多个茎尖分生组织是研究分生组织蛋白质组的理想模型。利用针对少量组织开发的二维电泳方案和基于质谱的跨物种多肽鉴定方法，我们揭示了茎尖分生组织蛋白质组，并研究了蔗糖介导的渗透胁迫对一个耐旱品种的影响。通过非参数单变量统计对因高蔗糖处理而显著上调或下调的蛋白质进行分类。我们的结果表明，维持具有渗透保护作用的细胞内蔗糖浓度、增强节能糖酵解特定基因的表达以及保持细胞壁完整性对于维持体内平衡、适应脱水和在脱水状态下存活至关重要。通过将耐旱品种与脱水敏感品种进行比较，我们能够区分几种基因型特异性蛋白质（异构体），并将耐旱品种与参与能量代谢的蛋白质（如磷酸甘油酸激酶、磷酸葡萄糖变位酶、UDP-葡萄糖焦磷酸化酶）以及与胁迫适应相关的蛋白质（如类OSR40蛋白、类脱落酸胁迫成熟蛋白）联系起来。这项工作表明，蛋白质组分析可以成功地用于进行定量差异分析，并表征难处理作物中的遗传变异。
