Segarra Guillem, Casanova Eva, Bellido David, Odena Maria Antonia, Oliveira Eliandre, Trillas Isabel
Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Barcelona, Catalonia, Spain.
Proteomics. 2007 Nov;7(21):3943-52. doi: 10.1002/pmic.200700173.
Trichoderma spp. is one of the most commonly used biological control agents against plant pathogens. This fungus produces changes in plant metabolism, thus increasing growth and enhancing resistance to biotic and abiotic stresses. However, its modes of action remain to be defined. In the first hours of interaction between cucumber plant roots and Trichoderma asperellum strain T34, salicylic and jasmonic acid levels and typical antipathogenic peroxidase activity increase in the cotyledons to different degrees depending on the applied concentration of the fungi. The use of 2-DE protein profiling and MS analysis allowed us to identify 28 proteins whose expression was affected in cotyledons after cucumber root colonization by Trichoderma applied at high concentrations: 17 were found to be up-regulated while 11 were down-regulated. Proteins involved in ROS scavenging, stress response, isoprenoid and ethylene biosynthesis, and in photosynthesis, photorespiration, and carbohydrate metabolism were differentially regulated by Trichoderma. The proteome changes found in this study help to give an understanding of how Trichoderma-treated plants become more resistant to pathogen attacks through the changes in expression of a set of defence-oriented proteins which can directly protect the plant or switch the metabolism to a defensive, nonassimilatory state.
木霉菌是最常用于防治植物病原体的生物防治剂之一。这种真菌会引起植物新陈代谢的变化，从而促进生长并增强对生物和非生物胁迫的抗性。然而，其作用方式仍有待确定。在黄瓜植株根系与棘孢木霉菌株T34相互作用的最初几个小时内，子叶中的水杨酸和茉莉酸水平以及典型的抗病原过氧化物酶活性会根据所施用真菌的浓度不同程度地增加。使用双向电泳蛋白质谱分析和质谱分析，我们鉴定出28种在高浓度木霉菌定殖黄瓜根系后子叶中表达受到影响的蛋白质：其中17种被上调，11种被下调。参与活性氧清除、应激反应、类异戊二烯和乙烯生物合成以及光合作用、光呼吸和碳水化合物代谢的蛋白质受到木霉菌的差异调节。本研究中发现的蛋白质组变化有助于理解经木霉菌处理的植物如何通过一组防御导向型蛋白质表达的变化变得更能抵抗病原体攻击，这些蛋白质可以直接保护植物或将新陈代谢转变为防御性的、非同化状态。
