Trifilieff E, Dubs M C, Van Regenmortel M H
Laboratoire d'Immunochimie, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Mol Immunol. 1991 Aug;28(8):889-96. doi: 10.1016/0161-5890(91)90053-m.
A number of continuous epitopes of tobacco mosaic virus protein (TMVP) have been defined by the pepscan technique using polyclonal and monoclonal antibodies to TMVP as well as antisera raised against synthetic peptides. In general, the location of continuous epitopes agreed with the results of earlier studies with peptides synthesized by classical methods although there were some notable exceptions. Results obtained with the different types of antibodies used in this study indicated that a homology of three residues was sufficient to give rise to antigenic cross-reactions. In the case of antibodies raised against a peptide conjugated to ovalbumin, some unexpected cross-reactivities could be explained by assuming that antibodies to the carrier molecule recognized homologous tripeptide sequences in TMVP and ovalbumin.
利用针对烟草花叶病毒蛋白（TMVP）的多克隆抗体和单克隆抗体以及针对合成肽产生的抗血清，通过肽扫描技术已确定了TMVP的一些连续表位。总体而言，连续表位的位置与早期用经典方法合成肽的研究结果一致，不过也有一些显著的例外情况。本研究中使用的不同类型抗体所获得的结果表明，三个残基的同源性足以引发抗原交叉反应。对于针对与卵清蛋白偶联的肽产生的抗体，一些意外的交叉反应性可以通过假设针对载体分子的抗体识别TMVP和卵清蛋白中的同源三肽序列来解释。
