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Monoclonal antibodies reactive with specific amino acid sequences of the 126 K protein of tobacco mosaic virus.

作者信息

Das P, Hari V

机构信息

Department of Biological Sciences, Wayne State University, Detroit, Michigan.

出版信息

Arch Virol. 1994;137(1-2):179-83. doi: 10.1007/BF01311185.

DOI:10.1007/BF01311185
PMID:7526825
Abstract

The 126 K protein of tobacco mosaic virus (TMV) is an NTP binding protein that has guanylyl transferase activity and is predicted to be an ATPase/helicase. In this paper we report the generation of monoclonal antibodies (Mabs) that react with specific amino acid sequences of the 126K protein. The Mabs were generated after immunizing mice with a partially purified preparation of the 126 K protein (native) obtained by centrifugal fractionation of the infected tissue extracts. The Mabs were assayed for specific reactivity by western blotting and by their reactivity with non-overlapping decapeptides corresponding to the entire amino acid sequence of the 126 K protein of TMV. A total of 11 Mabs reactive with specific peptides and three other Mabs that did not react with any peptide but reacted with the 126 K protein were identified.

摘要

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本文引用的文献

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An analysis of tobacco mosaic virus replicative structures synthesized in vitro.体外合成的烟草花叶病毒复制结构分析。
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An NTP-binding motif is the most conserved sequence in a highly diverged monophyletic group of proteins involved in positive strand RNA viral replication.NTP 结合基序是参与正链 RNA 病毒复制的高度分化单系蛋白组中最保守的序列。
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