利用合成肽鉴定肠道病毒结构蛋白和非结构蛋白中的组共同线性表位
Identification of group-common linear epitopes in structural and nonstructural proteins of enteroviruses by using synthetic peptides.
作者信息
Cello J, Samuelson A, Stålhandske P, Svennerholm B, Jeansson S, Forsgren M
机构信息
Department of Clinical Virology, University of Göteborg, Sweden.
出版信息
J Clin Microbiol. 1993 Apr;31(4):911-6. doi: 10.1128/jcm.31.4.911-916.1993.
Abstract
Synthetic peptides were employed in enzyme-linked immunosorbent assays to identify group-common linear epitopes in the structural and nonstructural proteins of enteroviruses. Nine linear epitopes were recognized by using sera from patients with heterotypic immunoglobulin G antibody responses to enterovirus infections. The most-reactive peptides were derived from conserved regions of the amino-terminal part of VP1, whereas peptides representing sequences from other conserved regions of VP1, as well as VP2, VP3, and VP4, and from a nonstructural region showed no or poor reactivity. These findings may be useful in the development of serological tests for the diagnosis of infections caused by a broad range of enteroviruses.
摘要
合成肽被用于酶联免疫吸附测定，以鉴定肠道病毒结构蛋白和非结构蛋白中的组共同线性表位。利用对肠道病毒感染有异型免疫球蛋白G抗体反应的患者血清识别出9个线性表位。反应性最强的肽来自VP1氨基末端部分的保守区域，而代表VP1其他保守区域以及VP2、VP3和VP4的序列以及来自非结构区域的肽则无反应或反应性差。这些发现可能有助于开发用于诊断多种肠道病毒引起的感染的血清学检测方法。
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