Ochi Yuji, Okamoto Akimitsu, Saito Isao
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):73-4. doi: 10.1093/nass/48.1.73.
Two pyrene-labeled bases, (py)C and (8py)G, have been synthesized and incorporated into d(CG)n. In the CD spectra, structural transition of a modified oligodeoxynucleotide (ODN-Py) from B-form to Z-form has been observed under different salt concentrations. The conformational transition of ODN-Py was monitored by the change of emission wavelengths based on exciplex and monomer fluorescence of (py)C and (8py)G.
已合成了两个芘标记的碱基，即（芘）胞嘧啶（(py)C）和（8-芘）鸟嘌呤（(8py)G），并将它们掺入到d(CG)n中。在圆二色光谱中，已观察到在不同盐浓度下，修饰的寡脱氧核苷酸（ODN-Py）从B型向Z型的结构转变。基于（芘）胞嘧啶（(py)C）和（8-芘）鸟嘌呤（(8py)G）的激基复合物和单体荧光，通过发射波长的变化监测了ODN-Py的构象转变。
