从极端嗜盐古菌日本嗜盐嗜盐菌TR-1菌株中分离crtI同源物。

Isolation of crtI homolog from extremely halophilic archaeon Haloarcula japonica strain TR-1.

作者信息

Yatsunami Rie, Takaichi Shinichi, Nakamura Satoshi

机构信息

Department of Bioengineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):193-4. doi: 10.1093/nass/48.1.193.

DOI:10.1093/nass/48.1.193

PMID:17150544

Abstract

Halobacterium sp. strain NRC-1 possesses phytoene dehydrogenase genes (crtIs). A crtI2 homolog was cloned from Haloarcula japonica strain TR-1. Nucleotide sequencing analysis of the possible crtI2 revealed that the structural gene consisted of an open reading frame of 1,521 nucleotides encoding 507 amino acids. Transcription of crtI2 in Ha. japonica was confirmed by RT-PCR.

摘要

嗜盐杆菌属菌株NRC-1拥有八氢番茄红素脱氢酶基因（crtIs）。从日本嗜盐嗜碱菌菌株TR-1中克隆出了一个crtI2同源物。对可能的crtI2进行核苷酸测序分析表明，该结构基因由一个1521个核苷酸的开放阅读框组成，编码507个氨基酸。通过逆转录聚合酶链反应证实了crtI2在日本嗜盐嗜碱菌中的转录。