日本嗜盐嗜碱菌TR-1株细胞表面糖蛋白编码基因的结构与异源表达
Structure and heterologous expression of the gene encoding the cell surface glycoprotein from Haloarcula japonica strain TR-1.
作者信息
Wakai H, Takada K, Nakamura S, Horikoshi K
机构信息
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Nucleic Acids Symp Ser. 1995(34):101-2.
The gene encoding the cell surface glycoprotein (CSG) of Haloarcula japonica strain TR-1 was cloned and sequenced. The structural gene consisted from an open reading frame of 2,586 bp. A potential promoter sequence was found about 150 bp upstream of the ATG initiation codon. N-terminal amino acid sequence of the Ha. japonica CSG revealed that the mature CSG consisted of 828 amino acids. Five potential N-glycosylation sites were found in the mature sequence. The cloned CSG gene of Ha. japonica was expressed in closely-related halophilic archaea.
克隆并测序了日本嗜盐嗜碱菌TR-1菌株细胞表面糖蛋白（CSG）的编码基因。结构基因由一个2586 bp的开放阅读框组成。在ATG起始密码子上游约150 bp处发现了一个潜在的启动子序列。日本嗜盐嗜碱菌CSG的N端氨基酸序列显示，成熟的CSG由828个氨基酸组成。在成熟序列中发现了五个潜在的N-糖基化位点。克隆的日本嗜盐嗜碱菌CSG基因在密切相关的嗜盐古菌中表达。
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